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Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein

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Abstract

Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.

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Acknowledgments

This work was supported by National Natural Science Foundation of China Grants 30801379 (G.C. Li), 30800093 (G.C. Li), 30470745 (W.B. Qian).

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Authors and Affiliations

  1. Xin Yuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People’s Republic of China

    Gong Chu Li, Na Li, Yan Hong Zhang, Xin Li, Yi Gang Wang & Xin Yuan Liu

  2. Department of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of China

    Wen Bin Qian

  3. Institute of Bioengineering, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People’s Republic of China

    Xiao Chuan Liu

  4. Division of Gene Therapy, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, People’s Republic of China

    Xin Yuan Liu

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  1. Gong Chu Li
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  4. Xin Li
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Corresponding authors

Correspondence to Wen Bin Qian or Xiao Chuan Liu.

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Gong Chu Li and Na Li contributed equally to this work.

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Li, G.C., Li, N., Zhang, Y.H. et al. Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein. Int J Hematol 89, 611–617 (2009). https://doi.org/10.1007/s12185-009-0308-3

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  • DOI: https://doi.org/10.1007/s12185-009-0308-3

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