Abstract
Ochratoxin A (OTA) is considered as one of the most hazardous mycotoxins commonly occurring in grains. Thus, the monitoring of OTA is extremely significant. Herein, a synergistic strategy to detect OTA in grains based on the combination of DNA tetrahedron-structured aptamer (DTA) and hybridization chain reaction (HCR) was proposed. Briefly, DTA was assembled on the gold electrode. In the presence of OTA, the trigger DNA on the top of the DTA was exposed to unfold the biotin-modified hairpin structure DNA to HCR. It could conjugate plentiful streptavidin-labeled horseradish peroxidase (SA-HRP) to form polyaniline (PANI), which was regarded as electrochemical active molecule to detect OTA. In this way, the synergistic effect of DTA and HCR not only can provide stable probe structure to improve the recognition rate of OTA, but also can supply a large number of templates to synthesize PANI under mild conditions. As a result, the biosensor has a good analytical performance for OTA in a linear range of 0.05 to 50 ng mL−1, with a detection limit of 0.017 ng mL−1. The accuracy of sensor is similar with high-performance liquid chromatography (HPLC) analysis.
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Funding
This work was financially supported by the National Key Research and Development Program of China (No. 2018YFC1602800), the National Natural Science Foundation of China (No. 21804071), the Natural Science Foundation of Jiangsu Province of China (No. BK20180688), and the University-Industry Collaborative Education Program (201901102022).
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Wei Yuan and Lixia Lu: Data curation, Writing — original draft. Yichen Lu, Xiong Xiong, and Yi Li: Conceptualization, Methodology, Writing — review & editing. Xiaowen Cui and Yuanjian Liu: Funding acquisition, Project administration, Writing — review & editing. Yuanjian Liu and Xiaohui Xiong: Formal analysis, Software, Supervision.
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Wei Yuan declares that he has no conflict of interest. Lixia Lu declares that he has no conflict of interest. Yichen Lu declares that he has no conflict of interest. Xiong Xiong declares that he has no conflict of interest. Yi Li declares that he has no conflict of interest. Xiaowen Cui declares that he has no conflict of interest. Yuanjian Liu declares that he has no conflict of interest. Xiaohui Xiong declares that he has no conflict of interest.
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Yuan, W., Lu, L., Lu, Y. et al. Synergistic Effects of DNA Structure for Ultrasensitive Detecting OTA in Grains. Food Anal. Methods 14, 2308–2316 (2021). https://doi.org/10.1007/s12161-021-02060-x
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DOI: https://doi.org/10.1007/s12161-021-02060-x