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Lipidomics Strategy for the Identification of Ultra-High Temperature and Reconstituted Milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry

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Abstract

Lipidomic strategies have been applied to many methodologies, such as species differentiation and lipid-associated disease identification. In this study, we first applied a lipidomic strategy coupled with UPLC-Q-Exactive Orbitrap mass spectrometry to investigate the differences between reconstituted milk and ultra-high temperature sterilization (UHT) milk. Fifteen lipids were selected as biomarkers to identify UHT and reconstituted milk by OPLS-DA method. Furthermore, a discriminant model to identify reconstituted milk from UHT milk was established based on these biomarkers. This robust discriminative model provided a strong technical proof to promote the development of the market norms concerning the identification of UHT and reconstituted milk.

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Abbreviations

Cer:

Ceramide

DG:

Diglyceride

GlcCer:

Glucosylceramide

LPC:

Lysophosphatidylcholine

LPE:

Lysophosphatidylethanolamine

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PA:

Phosphatidic acid

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

PG:

Phosphatidylglycerol

SM:

Sphingomyelin

TG:

Triglyceride

LA:

Linoleic acid

ARA:

Arachidonic acid

ALA:

α-linolenic acid

MG:

Monoglyceride

So:

Sphingosine

WE:

Wax ester

CL:

Cardiolipin

CerP:

Ceramide phosphate

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Funding

This work was supported by the National Key R&D Program of China (No. 2018YFC1602300) and the Natural Science Foundation of China (31871891).

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Authors and Affiliations

  1. Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China

    Jing Cui, Meicheng Su, Dongfei Tan, Xia Zhang, Man Jia & Gang Chen

  2. Chemistry Department, University of Otago, Dunedin, 9016, New Zealand

    Dan Zhu

  3. R&D Center, New Hope Dairy Co, Ltd., Chengdu, 610000, China

    Huaxing Wu

  4. Dairy Nutrition and Function, Key Laboratory of Sichuan Province, Chengdu, 610000, China

    Huaxing Wu

Authors
  1. Jing Cui
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  2. Dan Zhu
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  3. Meicheng Su
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  4. Dongfei Tan
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  5. Xia Zhang
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  6. Man Jia
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  7. Huaxing Wu
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  8. Gang Chen
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Correspondence to Gang Chen.

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Jing Cui declares that she has no conflict of interest. Dan Zhu declares that she has no conflict of interest. Meicheng Su declares that she has no conflict of interest. Dongfei Tan declares that he has no conflict of interest. Xia Zhang declares that she has no conflict of interest. Man Jia declares that she has no conflict of interest. Gang Chen declares that he has no conflict of interest.

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Cui, J., Zhu, D., Su, M. et al. Lipidomics Strategy for the Identification of Ultra-High Temperature and Reconstituted Milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry. Food Anal. Methods 14, 1064–1073 (2021). https://doi.org/10.1007/s12161-020-01947-5

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