Abstract
QuEChERS combined with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed to analyze multi-class mycotoxins including aflatoxins, ochratoxins, and Alternaria toxins in apples and tomatoes. Using apples and tomatoes as the research models, the method performance had been evaluated, showing great convenience, high sensitivity, and good reliability. The limit of detections was of 0.05–20 μg/L. The average recovery values were from 71.3 to 119.4%. With respect to precision, the repeatability was lower than 14.4%. With the optimum method, risk monitoring of mycotoxins in fresh and stored apples and tomatoes was executed, which indicated the risk of contamination of Alternaria toxins in apples which were preserved over a period of time.
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This work was supported by The National Key Research and Development Program of China (2017YFC1601600).
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Yan Tang declares no conflict of interest. Lei Mu declares no conflict of interest. Jiaxing Cheng declares no conflict of interest. Zhenxia Du declares no conflict of interest. Youyou Yang declares no conflict of interest.
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Highlight
• A QuEChERS method coupled with UHPLC-MS/MS was developed for multi-class mycotoxin analysis.
• The proposed method is robust as well as sensitive for determination of eleven mycotoxins.
• The methodology is applicable to routinely monitor the eleven mycotoxins in apples and tomatoes.
• AME was detected in tomato samples, and ALT, ALT-I, AOH, and AME were detected in apple samples
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Tang, Y., Mu, L., Cheng, J. et al. Determination of Multi-Class Mycotoxins in Apples and Tomatoes by Combined Use of QuEChERS Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 13, 1381–1390 (2020). https://doi.org/10.1007/s12161-020-01753-z
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DOI: https://doi.org/10.1007/s12161-020-01753-z