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Amperometric Photosensor Based on Acridine Orange/TiO2 for Chlorogenic Acid Determination in Food Samples

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Abstract

The authors describe a novel sensor for chlorogenic acid (CGA) detection/quantification in food samples. The photosensor is based on a composite of titanium dioxide (TiO2) and acridine orange (AO). The synergism between AO and TiO2 was revealed under visible LED light irradiation by high photocurrents for CGA, when compared to each component of the composite material. A detection limit of 0.54 μmol L−1 and a linear response range from 2 to 200 μmol L−1 for CGA detection were achieved. The selectivity of the sensor was tested by using common interferents on samples containing chlorogenic acid and results suggested that there is no significant interference in the analyte response, indicating a good selectivity. Finally, the photosensor was successfully applied in the determination of CGA in samples of coffee, tea, and apple juice, with recoveries ranging from 100.9 to 102.4%, suggesting a good accuracy for the proposed method.

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Funding

This work was supported by Fundação de Amparo à Pesquisa do Estado do Maranhão (00155/16; 00927/16; 01194/17), Conselho Nacional de Desenvolvimento Científico e Tecnológico (303525/2016-9; 421139/2016-1; 305680/2015-3; 426337/2016-6), and Instituto Nacional de Ciência e Tecnologia em Bioanalítica (465389/2014-7). The authors gratefully acknowledge the Microscopy Centre/UFMG that provided the images.

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Authors and Affiliations

  1. Department of Chemistry, Federal University of Maranhão, São Luís, MA, 65080-805, Brazil

    Kelline Alaíde Pereira Sousa, Fernanda Maria Rodrigues Lima, Thatyara Oliveira Monteiro, Flavio Santos Damos & Rita de Cássia Silva Luz

  2. School of Chemistry, University of New South Wales, Sydney, 2052, Australia

    Saimon Moraes Silva

  3. Federal University of Alagoas, Av. Lourival Melo Mota s/n, Maceió, AL, 57072-970, Brazil

    Marília Oliveira Fonseca Goulart

Authors
  1. Kelline Alaíde Pereira Sousa
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  2. Fernanda Maria Rodrigues Lima
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  3. Thatyara Oliveira Monteiro
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  4. Saimon Moraes Silva
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  5. Marília Oliveira Fonseca Goulart
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  6. Flavio Santos Damos
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  7. Rita de Cássia Silva Luz
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Correspondence to Rita de Cássia Silva Luz.

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Conflict of Interest

K.A.P. Sousa declares that she has no conflict of interest. F.M.R. Lima declares that she has no conflict of interest. T.O. Monteiro declares that she has no conflict of interest. S.M. Silva declares that he has no conflict of interest. M.O.F. Goulart declares that she has no conflict of interest. F.S. Damos declares that he has no conflict of interest. R.C.S. Luz declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Sousa, K.A.P., Lima, F.M.R., Monteiro, T.O. et al. Amperometric Photosensor Based on Acridine Orange/TiO2 for Chlorogenic Acid Determination in Food Samples. Food Anal. Methods 11, 2731–2741 (2018). https://doi.org/10.1007/s12161-018-1261-7

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  • DOI: https://doi.org/10.1007/s12161-018-1261-7

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