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Simultaneous Determination of Cyclamate, Acesulfame, and Aspartame in Beverages by Titania-Based RP-HPLC

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Abstract

This paper aims at establishing a rapid method for simultaneous separation and determination of the sweeteners including cyclamate, acesulfame, and aspartame in beverages by titania-based reversed-phase high-performance liquid chromatography. The chromatographic conditions were as follows: a titania Sachtopore-RP C18 column (250 × 4.6 mm; 5 μm) as a separation column, a mixture of water and methanol at a volume ratio of 95:5 containing 1.0 % phosphate acid used as mobile phase, flow rate of 1.0 mL min−1, and the detection wavelength was 205 nm. The linear ranges of cyclamate and aspartame were 0.02–8.0 mg mL−1 with limits of detection (LODs) of 16.35 and 19.56 μg mL−1, respectively, and their limits of quantitation (LOQs) were 55.50 and 68.50 μg mL−1, respectively. The recoveries of cyclamate were between 93.52 and 103.54 %. The recoveries of aspartame were between 93.31 and 102.63 %. The linear range of acesulfame was 0.125–50 μg mL−1 with LOD of 0.08 μg mL−1 and LOQ of 0.25 μg mL−1. The recoveries of acesulfame were between 94.34 and 103.21 %. Relative standard deviation (n = 8) for all determinations was less than 0.72 %.

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References

  • Cang GQ, Lu Q, Cheng C, Shao H (2011) The comparative study of ion chromatography and gas chromatography for determination of sodium cyclamate. Chin J Health Lab Technol 21(9):2338–2339

    Google Scholar 

  • Chen SB, Pan CH (2000) Determination of sodium cyclamate in foods by ultraviolet spectrophotometry. J Ins Anal 19(3):82–83

    CAS  Google Scholar 

  • Chen QC, Mou SF, Liu KN, Yang ZY, Ni ZM (1997) Separation and determination of four artificial sweeteners and citric acid by high-performance anion-exchange chromatography. J Chromatogr A 771:135–143

    Article  CAS  Google Scholar 

  • Chen Y, Yi Y, Brennan JD, Brook MA (2006) Development of macroporous titania monoliths using a biocompatible method. Part 1: Material fabrication and characterization. Chem Mater 18:5326–5335

    Article  CAS  Google Scholar 

  • Chinese National Standard (2011) GB2760-2011: hygiene standards for use of food additives. China Standard Press, Beijing

    Google Scholar 

  • George V, Arora S, Wadhwa BK, Singh AK (2010) Analysis of multiple sweeteners and their degradation products in lassi by HPLC and HPTLC plates. J Food Sci Technol 47(4):408–413

    Article  CAS  Google Scholar 

  • Hao YP (2012) Application of spectrophotometry in detection of food additive content. Agric Prod Process 5:74–75

    Google Scholar 

  • Horie M, Ishikawa F, Oishi M, Shindo T, Yasui A, Ito K (2007) Rapid determination of cyclamate in foods by solid-phase extraction and capillary electrophoresis. J Chromatogr A 1154(1–2):423–428

    Article  CAS  Google Scholar 

  • Jiang B, Feng ZB (2005) Research progress of sweeteners. Shanxi Food Ind 3:16–19

    Google Scholar 

  • Kritsunankul O, Jakmunee J (2011) Simultaneous determination of some food additives in soft drinks and other liquid foods by flow injection on-line dialysis coupled to high performance liquid chromatography. Talanta 84:1342–1349

    Article  CAS  Google Scholar 

  • Kuroda I, Shintani Y, Motokawa M, Abe S, Furuno M (2004) Phosphopeptide-selective column-switching RP-HPLC with a titania precolumn. Anal Sci 20:1313–1319

    Article  CAS  Google Scholar 

  • Liu WH, Guo YY (2000) Thin-layer chromatography for the simultaneous determination of sodium cyclamate and sodium saccharin in beverages. J Chin Pub Health 16(8):766

    Google Scholar 

  • Lu L, Hang YP, Gao YH (2009) Rapid determination of common sweeteners in milk beverage by HPLC. Food Sci 30(10):166–168

    CAS  Google Scholar 

  • Qu F, Zhu HQ, Liu KN, Mou SF (1999) Determination of aspartame by ion chromatography with electrochemical integrated amperometric detection. J Chromatogr A 850(1–2):277–281

    Article  CAS  Google Scholar 

  • Randon J, Guerrin JF, Rocca JL (2008) Synthesis of titania monoliths for chromatographic separations. J Chromatogr A 1214:183–186

    Article  CAS  Google Scholar 

  • Serdar M, Knezevic Z (2011) Determination of artificial sweeteners in beverages and special nutritional products using high performance liquid chromatography. Arh Hig Rada Toksikol 62:169–173

    Article  CAS  Google Scholar 

  • Sik B (2012) Development and validation of a green high performance liquid chromatographic method for the determination of some artificial sweeteners and caffeine in soft drinks. Food Anal Methods 5:1443–1452

    Article  Google Scholar 

  • Tani K, Kitada M, Tachibana M, Koizumi H, Kiba T (2003) Retention behavior of monosaccharides and disaccharideson titania. Chromatographia 57:409–412

    Article  CAS  Google Scholar 

  • Wang FE, Yang GS, Tan L (2011) Research progress of chromatography detection of artificial sweeteners. Shandong Agric Sci 12:93–96

    Google Scholar 

  • Wasik A, Mccourt J, Buchgraber M (2007) Simultaneous determination of nine intense sweeteners in foodstuffs by high performance liquid chromatography and evaporative light scattering detection-development and single-laboratory validation. J Chromatogr A 1157:187–196

    Article  CAS  Google Scholar 

  • Xia YL, Li MC, Zhang Y, He KR, Yin LG, Wang T (2011) Simultaneous determination of four sweeteners in Chinese liquors by liquid chromatography-mass spectrometry. China Brewing 228(3):156–158

    Google Scholar 

  • Yu SL (2005) High performance liquid chromatography (HPLC) method and application, 2nd edn. Chemical Industry Press, Beijing

    Google Scholar 

  • Zhang H, Wang JD, Zhong HM, Luo LM (1998) Study of protein separation by reversed-phase high performance liquid chromatography. Chin J Chromatogr 16(3):220–222

    CAS  Google Scholar 

  • Zhao YL, Li HR, Han SZ, Han WA (2007) Research status and the trend of development of sweetness agent. Hebei Chem 30(7):25–28

    CAS  Google Scholar 

  • Zhao J, Jiang ZT, Lu GR, Tan J, Li R (2010) Determination of phosphatidylcholine in soybean lecithin samples by high performance liquid chromatography on titania. Anal Methods 2:1779–1783

    Article  CAS  Google Scholar 

  • Zhong ZX, Liang CH, Yao J, Fan SH, Xu XM (2005) Determination of sodium cyclamate, benzoic acid in food by ion chromatography. Chin J Health Lab Technol 15(9):1062–1064

    CAS  Google Scholar 

  • Zhu Y, Guo YY, Ye ML, James FS (2005) Separation and simultaneous determination of four artificial sweeteners in food and beverages by ion chromatography. J Chromatogr A 1085:143–146

    Article  CAS  Google Scholar 

  • Zizkovsky V, Kucera R, Klimes J, Dohnal J (2008) Titania-based tationary phase in separation of Ondansetron and its related compounds. J Chromatogr A 1189:83–91

    Article  CAS  Google Scholar 

  • Zygler A, Wasik A, Namiesnik J (2009) Analytical methodologies for determination of artificial sweeteners in foodstuffs. Trends in Anal Chem 28(9):1082–1102

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Natural Science Foundation of Tianjin (grant 13JCYBJC18700) and the Science Foundation for Young Teachers of Tianjin University of Commerce (grant 090107).

Conflict of Interest

Tenghao Liu declares that she has no conflict of interest. Zi-Tao Jiang declares that he has no conflict of interest. Rong Li declares that she has no conflict of interest. Jin Tan declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Correspondence to Zi-Tao Jiang.

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Liu, T., Jiang, ZT., Li, R. et al. Simultaneous Determination of Cyclamate, Acesulfame, and Aspartame in Beverages by Titania-Based RP-HPLC. Food Anal. Methods 7, 1400–1406 (2014). https://doi.org/10.1007/s12161-013-9762-x

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  • DOI: https://doi.org/10.1007/s12161-013-9762-x

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