Abstract
This paper aims at establishing a rapid method for simultaneous separation and determination of the sweeteners including cyclamate, acesulfame, and aspartame in beverages by titania-based reversed-phase high-performance liquid chromatography. The chromatographic conditions were as follows: a titania Sachtopore-RP C18 column (250 × 4.6 mm; 5 μm) as a separation column, a mixture of water and methanol at a volume ratio of 95:5 containing 1.0 % phosphate acid used as mobile phase, flow rate of 1.0 mL min−1, and the detection wavelength was 205 nm. The linear ranges of cyclamate and aspartame were 0.02–8.0 mg mL−1 with limits of detection (LODs) of 16.35 and 19.56 μg mL−1, respectively, and their limits of quantitation (LOQs) were 55.50 and 68.50 μg mL−1, respectively. The recoveries of cyclamate were between 93.52 and 103.54 %. The recoveries of aspartame were between 93.31 and 102.63 %. The linear range of acesulfame was 0.125–50 μg mL−1 with LOD of 0.08 μg mL−1 and LOQ of 0.25 μg mL−1. The recoveries of acesulfame were between 94.34 and 103.21 %. Relative standard deviation (n = 8) for all determinations was less than 0.72 %.
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This work was supported by the Natural Science Foundation of Tianjin (grant 13JCYBJC18700) and the Science Foundation for Young Teachers of Tianjin University of Commerce (grant 090107).
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Tenghao Liu declares that she has no conflict of interest. Zi-Tao Jiang declares that he has no conflict of interest. Rong Li declares that she has no conflict of interest. Jin Tan declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.
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Liu, T., Jiang, ZT., Li, R. et al. Simultaneous Determination of Cyclamate, Acesulfame, and Aspartame in Beverages by Titania-Based RP-HPLC. Food Anal. Methods 7, 1400–1406 (2014). https://doi.org/10.1007/s12161-013-9762-x
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DOI: https://doi.org/10.1007/s12161-013-9762-x