Abstract
The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol L−1 borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg L−1 and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg L−1, for vitamins PP (nicotinamide), B12 (cyanocobalamin), B2 (riboflavin), B6 (pyridoxine), B8 (biotin), C (ascorbic acid), B5 (pantothenic acid), B3 (nicotinic acid), B1 (thiamine), and B9 (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods.
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The authors are grateful to CAPES, CNPq, FINEP, and Fundação Araucária for the financial support.
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Danielle C. da Silva declares that he has no conflict of interest. Jesuí V. Visentainer declares that he has no conflict of interest. Nilson E. de Souza declares that he has no conflict of interest. Cláudio C. Oliveira declares that he has no conflict of interest. The article does not contain any studies with human or animal subjects and the authors not have a financial relationship with the organization that sponsored the research. The financial support came from the Public Brazilian Agencies that do not have commercial interest.
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da Silva, D.C., Visentainer, J.V., de Souza, N.E. et al. Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements. Food Anal. Methods 6, 1592–1606 (2013). https://doi.org/10.1007/s12161-013-9576-x
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DOI: https://doi.org/10.1007/s12161-013-9576-x