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Evaluation of Dispersive Liquid–Liquid Microextraction–HPLC–UV for Determination of Deoxynivalenol (DON) in Wheat Flour

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Abstract

A fast and simple method for the extraction of deoxynivalenol (DON) from wheat flour using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography–UV detection has been developed and compared with immunoaffinity column cleanup (IAC) process. The influence of several important parameters on the extraction efficacy was studied. Under optimized conditions, a linear calibration curve was obtained in the range of 50–1,000 μg/L. Average recoveries of DON from spiked wheat samples at levels of 500 μg/kg for DLLME and IAC ranged from 72.9 ± 1.6 and 85.5 ± 3.1, respectively. A good correlation was found for spiked samples between DLLME and IAC methods. The limit of detection was 125 and 50 μg/kg for DLLME and IAC method, respectively. Advantages of DLLME method with respect to the IAC have been pointed out.

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Authors and Affiliations

  1. Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran

    Rouhollah Karami-Osboo, Ramin Miri & Katayoun Javidnia

  2. Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

    Mehdi Maham

  3. Faroogh Life Sciences Research Laboratory No. 96.2, Tehran, 1457844393, Iran

    Mohammad Hossein Shojaee AliAbadi & Mansoureh Mirabolfathy

Authors
  1. Rouhollah Karami-Osboo
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  2. Mehdi Maham
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  3. Ramin Miri
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  4. Mohammad Hossein Shojaee AliAbadi
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  5. Mansoureh Mirabolfathy
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  6. Katayoun Javidnia
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Correspondence to Katayoun Javidnia.

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Karami-Osboo, R., Maham, M., Miri, R. et al. Evaluation of Dispersive Liquid–Liquid Microextraction–HPLC–UV for Determination of Deoxynivalenol (DON) in Wheat Flour. Food Anal. Methods 6, 176–180 (2013). https://doi.org/10.1007/s12161-012-9428-0

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  • DOI: https://doi.org/10.1007/s12161-012-9428-0

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