Abstract
Objective
Arginine–arginine–leucine (RRL) is considered a tumor endothelial cell-specific binding sequence. RRL-containing peptide targeting tumor vessels is an excellent candidate for tumor imaging. In this study, we developed RRL-containing hexapeptides and evaluated their feasibility as a tumor imaging agent in a HT-1080 fibrosarcoma-bearing murine model.
Methods
The hexapeptide, glutamic acid–cysteine–glycine (ECG)–RRL was synthesized using Fmoc solid-phase peptide synthesis. Radiolabeling efficiency was evaluated using instant thin-layer chromatography. Uptake of Tc-99m ECG–RRL within HT-1080 cells was evaluated in vitro by confocal microscopy and cellular binding affinity was calculated. Gamma images were acquired In HT-1080 fibrosarcoma tumor-bearing mice, and the tumor-to-muscle uptake ratio was calculated. The inflammatory-to-normal muscle uptake ratio was also calculated in an inflammation mouse model. A biodistribution study was performed to calculate %ID/g.
Results
A high yield of Tc-99m ECG–RRL complexes was prepared after Tc-99m radiolabeling. Binding of Tc-99m ECG–RRL to tumor cells had was confirmed by in vitro studies. Gamma camera imaging in the murine model showed that Tc-99m ECG–RRL accumulated substantially in the subcutaneously engrafted tumor and that tumoral uptake was blocked by co-injecting excess RRL. Moreover, Tc-99m ECG–RRL accumulated minimally in inflammatory lesions.
Conclusions
We successfully developed Tc-99m ECG–RRL as a new tumor imaging candidate. Specific tumoral uptake of Tc-99m ECG–RRL was evaluated both in vitro and in vivo, and it was determined to be a good tumor imaging candidate. Additionally, Tc-99m ECG–RRL effectively distinguished between cancerous tissue and inflammatory lesions.
Similar content being viewed by others
References
Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144:646–74.
Gladson CL, Cheresh DA. Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells. J Clin Invest. 1991;88:1924–32.
Veikkola T, Karkkainen M, Claesson-Welsh L, Alitalo K. Regulation of angiogenesis via vascular endothelial growth factor receptors. Cancer Res. 2000;60:203–12.
Bhagwat SV, Lahdenranta J, Giordano R, Arap W, Pasqualini R, Shapiro LH. CD13/APN is activated by angiogenic signals and is essential for capillary tube formation. Blood. 2001;97:652–9.
Kim DW, Kim WH, Kim MH, Kim CG. Novel Tc-99m labeled ELR-containing 6-mer peptides for tumor imaging in epidermoid carcinoma xenografts model: a pilot study. Ann Nucl Med. 2013;27:892–7.
Kim DW, Kim WH, Kim MH, Kim CG. Synthesis and evaluation of novel Tc-99m labeled NGR-containing hexapeptides as tumor imaging agents. J Label Comp Radiopharm. 2015;58:30–5.
Brown CK, Modzelewski RA, Johnson CS, Wong MK. A novel approach for the identification of unique tumor vasculature binding peptides using an E. coli peptide display library. Ann Surg Oncol. 2000;7:743–749.
Lu X, Yan P, Wang RF, Liu M, Yu MM, Zhang CL, et al. The further study on radioiodinated peptide Arg-Arg-Leu targeted to neovascularization as well as tumor cells in molecular tumor imaging. J Radioanal Nucl Chem. 2011;290:623–30.
Weller GE, Wong MK, Modzelewski RA, Lu E, Klibanov AL, Wagner WR, et al. Ultrasonic imaging of tumor angiogenesis using contrast microbubbles targeted via the tumor-binding peptide arginine–arginine–leucine. Cancer Res. 2005;65:533–9.
Yu M, Zhou H, Liu X, Huo Y, Zhu Y, Chen Y. Study on biodistribution and imaging of radioiodinated arginine–arginine–leucine peptide in nude mice bearing human prostate carcinoma. Ann Nucl Med. 2010;24:13–9.
Lu X, Yan P, Wang RF, Liu M, Yu MM, Zhang CL. Use of radioiodinated peptide Arg-Arg-Leu targeted to neovascularization as well as tumor cells in molecular tumor imaging. Chin J Cancer Res. 2012;24:52–9.
Zhao Q, Yan P, Yin L, Li L, Chen XQ, Ma C, et al. Validation study of (1)(3)(1)I-RRL: assessment of biodistribution, SPECT imaging and radiation dosimetry in mice. Mol Med Rep. 2013;7:1355–60.
Zhao Q, Yan P, Wang RF, Zhang CL, Li L, Yin L. A novel 99mTc-labeled molecular probe for tumor angiogenesis imaging in hepatoma xenografts model: a pilot study. PLoS One. 2013;8:e61043.
Lu X, Zhao L, Xue T, Zhang H. Technetium-99 m- Arg-Arg-Leu(g2), a modified peptide probe targeted to neovascularization in molecular tumor imaging. J BUON. 2013;18:1074–81.
Yao N, Yan P, Wang RF, Zhang CL, Ma C, Chen XQ, et al. Detection of pulmonary metastases with the novel radiolabeled molecular probe, (99m)Tc-RRL. Int J Clin Exp Med. 2015;8:1726–36.
Endo K, Oriuchi N, Higuchi T, Iida Y, Hanaoka H, Miyakubo M, et al. PET and PET/CT using 18F-FDG in the diagnosis and management of cancer patients. Int J Clin Oncol. 2006;11:286–96.
Kim DW, Park SA, Kim CG. Dual-time-point positron emission tomography findings of benign mediastinal fluorine-18-fluorodeoxyglucose uptake in tuberculosis-endemic region. Indian J Nucl Med. 2011;26:3–6.
Takamochi K, Yoshida J, Murakami K, Niho S, Ishii G, Nishimura M, et al. Pitfalls in lymph node staging with positron emission tomography in non-small cell lung cancer patients. Lung Cancer. 2005;47:235–42.
Kubota R, Kubota K, Yamada S, Tada M, Ido T, Tamahashi N. Microautoradiographic study for the differentiation of intratumoral macrophages, granulation tissues and cancer cells by the dynamics of fluorine-18-fluorodeoxyglucose uptake. J Nucl Med. 1994;35:104–12.
Wu C, Wei J, Gao K, Wang Y. Dibenzothiazoles as novel amyloid-imaging agents. Bioorg Med Chem. 2007;15:2789–96.
Liu S, Edwards DS. 99mTc-labeled small peptides as diagnostic radiopharmaceuticals. Chem Rev. 1999;99:2235–68.
van Waarde A, Jager PL, Ishiwata K, Dierckx RA, Elsinga PH. Comparison of sigma-ligands and metabolic PET tracers for differentiating tumor from inflammation. J Nucl Med. 2006;47:150–4.
Sugae S, Suzuki A, Takahashi N, Minamimoto R, Cheng C, Theeraladanon C, et al. Fluorine-18-labeled 5-fluorouracil is a useful radiotracer for differentiation of malignant tumors from inflammatory lesions. Ann Nucl Med. 2008;22:65–72.
Fazaeli Y, Jalilian A, Amini M, Ardaneh K, Rahiminejad A, Bolourinovin F, et al. Development of a 68 Ga-fluorinated porphyrin complex as a possible PET imaging agent. Nucl Med Mol Imaging. 2012;46:20–6.
Shin K-H, Park S-A, Kim S-Y, Lee S, Oh S, Kim J. Effect of animal condition and fluvoxamine on the result of [18F]N-3-fluoropropyl-2β-carbomethoxy-3β-(4-iodophenyl) nortropane ([18F]FP-CIT) PET study in mice. Nucl Med Mol Imaging. 2012;46:27–33.
Berger M, Gould MK, Barnett PG. The cost of positron emission tomography in six United States Veterans Affairs hospitals and two academic medical centers. AJR Am J Roentgenol. 2003;181:359–65.
Yang DJ, Kim EE, Inoue T. Targeted molecular imaging in oncology. Ann Nucl Med. 2006;20:1–11.
Acknowledgments
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2013R1A1A2059262).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Kim, DW., Kim, W.H., Kim, M.H. et al. Synthesis and evaluation of Tc-99m-labeled RRL-containing peptide as a non-invasive tumor imaging agent in a mouse fibrosarcoma model. Ann Nucl Med 29, 779–785 (2015). https://doi.org/10.1007/s12149-015-1002-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12149-015-1002-6