Abstract
As sessile organisms, plants need to counteract different biotic and abiotic stresses to survive. RNA interference provides natural immunity against various plant pathogens, especially against viral infections via inhibition of viral genome replication or translation. In plants, DRB3, a multi-domain protein containing two N-terminal dsRNA binding domains (dsRBD), plays a vital role in RNA-directed DNA methylation of the geminiviral genome. Additionally, DRB3 arrests the replication of the viral genome in the viral replication complex of RNA viruses through a mechanism that has yet to be fully deciphered. Therefore, as a first step towards exploring the structural details of DRB3, we present a nearly complete backbone and side chain assignment of the two N-terminal dsRBD domains.
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Data availability
Backbone and sidechain chemical shift assignments data for DRB3 dsRBD1 and DRB3 dsRBD2 have been deposited in the BMRB database with accession No. 52133 and 52151, respectively.
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Acknowledgements
The authors acknowledge the initial assistance of Mr. Ramdas Aute, Dr. Sai Chaitanya Chiliveri, Dr. Upasana Rai, Dr. Jaishri Rubina Das, and Ms. Richa Garg in cloning DRB3 domains from drb3 full-length gene.
Funding
This work was supported by the Council of Scientific and Industrial Research, Government of India, through a CSIR-FIRST grant (MLP161). JP acknowledges Ph.D. Fellowship (JRF and SRF) from the INSPIRE program of the Department of Science and Technology (DST), Government of India.
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MVD planned the study. MVD and JP designed and performed the experiments. Both MVD and JP wrote the manuscript.
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Paul, J., Deshmukh, M.V. Chemical shift assignment of dsRBD1 and dsRBD2 of Arabidopsis thaliana DRB3, an essential protein involved in RNAi-mediated antiviral defense. Biomol NMR Assign 18, 99–104 (2024). https://doi.org/10.1007/s12104-024-10174-6
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DOI: https://doi.org/10.1007/s12104-024-10174-6