Abstract
SarA is a global transcription regulator in S. aureus which regulates the expression of over 120 genes related to quorum sensing, biofilm synthesis, drug resistance and many other important physiological processes during host infection. SarA can bind to the promoter region of agr and other target genes to activate or repress the transcription. The crystal structure of SarA uncovered a MarR protein-like conformation with two symmetrical winged helix domains, while its DNA binding mechanism is still unknown. We have constructed a monomeric DNA binding domain of SarA (SarAΔN19) for the study of the interaction between SarA and DNA with NMR spectroscopy. Here, we report the 1H, 13C and 15N NMR assignment of SarAΔN19/DNA complex which is the first step towards further structure and function analysis.
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Data availability
The 1H, 13C, and 15N chemical shifts were deposited in the BioMagResBank (https://www.bmrb.wisc.edu.) with BMRB accession number 51,661. All other data generated or analysed during this study are included in this published article.
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Acknowledgements
NMR experiments were performed at the Beijing NMR Center of National Center for Protein Sciences, at Peking University. This work was supported by grant 2016YFA0501202 from the Ministry of Science and Technology of China and grant 31570734 from the National Natural Science Foundation of China. We thank Dr. Hongwei Li and Dr. Xiaogang Niu for their assistance in the NMR experiments.
Funding
This work was supported by grant 2016YFA0501202 from the Ministry of Science and Technology of China and grant 31570734 from the National Natural Science Foundation of China.
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D.F. and B.D. carried out the experiment and analyzed data. D.F. and B.X. wrote the manuscript. B.X. and X.D. supervised the project.
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Fu, D., Duan, B., Dong, X. et al. 1H, 13C, and 15N resonance assignments of SarA monomer from Staphylococcus aureus in complex with DNA. Biomol NMR Assign 17, 193–197 (2023). https://doi.org/10.1007/s12104-023-10140-8
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DOI: https://doi.org/10.1007/s12104-023-10140-8