Abstract
Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the backbone chemical shift assignments of the full length Dpo4 in its binary complex with a 14/14-mer DNA substrate. Upon DNA binding, several β-stranded regions in the isolated catalytic core and little finger/linker fragments of Dpo4 become more structured. This work serves as a foundation for our ongoing investigation of conformational dynamics of Dpo4 and future determination of the first solution structures of a DNA polymerase and its binary and ternary complexes.
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Acknowledgments
This work was supported by the National Institute of Health Grant R21ES024585 to both Z.S. and Z.W.
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Lee, E., Fowler, J.D., Suo, Z. et al. Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA. Biomol NMR Assign 11, 39–43 (2017). https://doi.org/10.1007/s12104-016-9717-4
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DOI: https://doi.org/10.1007/s12104-016-9717-4