Abstract
Cytosolic dNT-1 nucleotidase plays a key role in the homeostasis of pyrimidine deoxyribonucleotides in mammalian cells. The enzyme is responsible for the dephosphorylation of physiological substrates as well as nucleoside analogues that are used in antiviral and anticancer therapies, therefore selective inhibition of the dNT-1 nucleotidase activity may lead to an increase in efficacy of this type of therapeutic compounds. Here, we report the backbone 1H, 13C and 15N assignments for the 47 kDa dNT-1 dimer, which will be used for structural characterisation of dNT-1 complexes with small molecule inhibitors obtained through modification of pyrimidine nucleotide scaffolds or optimisation of successful binders obtained from the screening of fragment libraries.
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Acknowledgments
We would like to thank Dr Kirsty Lightbody-Jones for valuable comments and suggestions during the manuscript preparation and Dr Frederick Muskett (University of Leicester, UK) for the optimized pulse sequences for triple-resonance NMR experiments. This work was supported by the Ministry of Education of the Czech Republic—LK11205 (programme “NAVRAT”), GACR 03/09/0820, and projects RVO 61388963 and 68378050 awarded by the Academy of Sciences of the Czech Republic.
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Hnízda, A., Skleničková, R., Pachl, P. et al. Backbone resonance assignments of human cytosolic dNT-1 nucleotidase. Biomol NMR Assign 8, 425–428 (2014). https://doi.org/10.1007/s12104-013-9531-1
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DOI: https://doi.org/10.1007/s12104-013-9531-1