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1H, 13C, and 15N chemical shift assignments of neuronal calcium sensor protein, hippocalcin

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Abstract

Hippocalcin, a member of the neuronal calcium sensor (NCS) subclass of the calmodulin superfamily, serves as an important calcium sensor for the slow afterhyperpolarizing (sAHP) current in the hippocampus, which underlies some forms of learning and memory. Hippocalcin is also a calcium sensor for hippocampal long-term depression (LTD) and genetically linked to neurodegenerative diseases. We report NMR chemical shift assignments of Ca2+-free hippocalcin (BMRB no. 18627).

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References

  • Ames JB, Lim S (2012) Molecular structure and target recognition of neuronal calcium sensor proteins. Biochim Biophys Acta 1820:1205–1213

    Article  Google Scholar 

  • Ames JB, Tanaka T, Stryer L, Ikura M (1994) Secondary structure of myristoylated recoverin determined by three-dimensional heteronuclear NMR: implications for the calcium-myristoyl switch. Biochemistry 33:10743–10753

    Article  Google Scholar 

  • Ames JB, Tanaka T, Stryer L, Ikura M (1996) Portrait of a myristoyl switch protein. Curr Opin Struct Biol 6:432–438

    Article  Google Scholar 

  • Ames JB, Ishima R, Tanaka T, Gordon JI, Stryer L, Ikura M (1997) Molecular mechanics of calcium-myristoyl switches. Nature 389:198–202

    Article  ADS  Google Scholar 

  • Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L (1999) Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases. J Biol Chem 274:19329–19337

    Article  Google Scholar 

  • Bourne Y, Dannenberg J, Pollmann VV, Marchot P, Pongs O (2001) Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor1). J Biol Chem 276:11949–11955

    Article  Google Scholar 

  • Burgoyne RD (2007) Neuronal calcium sensor proteins: generating diversity in neuronal Ca2 + signalling. Nat Rev Neurosci 8:182–193

    Article  Google Scholar 

  • Dizhoor AM, Chen CK, Olshevskaya E, Sinelnikova VV, Phillipov P, Hurley JB (1993) Role of the acylated amino terminus of recoverin in Ca(2 +)-dependent membrane interaction. Science 259:829–832

    Article  ADS  Google Scholar 

  • Flaherty KM, Zozulya S, Stryer L, McKay DB (1993) Three-dimensional structure of recoverin, a calcium sensor in vision. Cell 75:709–716

    Article  Google Scholar 

  • Ikura M, Kay LE, Bax A (1990) A novel approach for sequential assignment of 1H, 13C, and 15 N spectra of proteins: heteronuclear triple-resonanc three-dimensional NMR spectroscopy. Application to calmodulin. Biochemistry 29:4659–4667

    Google Scholar 

  • Jo J, Son GH, Winters BL, Kim MJ, Whitcomb DJ, Dickinson BA, Lee YB, Futai K, Amici M, Sheng M, Collingridge GL, Cho K (2010) Muscarinic receptors induce LTD of NMDAR EPSCs via a mechanism involving hippocalcin, AP2 and PSD-95. Nat Neurosci 13:1216–1224

    Article  Google Scholar 

  • Kobayashi M, Takamatsu K, Saitoh S, Miura M, Noguchi T (1993) Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus. Biochem Biophys Res Commun 196:1017

    Article  Google Scholar 

  • Lim, S., Strahl, T., Thorner, J. and Ames, J.B. (2011) Structure of a Ca2 +— myristoyl switch protein that controls activation of a phosphatidylinositol 4-kinase in fission yeast. J Biol Chem 286:12565–12577

    Google Scholar 

  • O’Callaghan DW, Tepikin AV, Burgoyne RD (2003) Dynamics and calcium sensitivity of the Ca2 +/myristoyl switch protein hippocalcin in living cells. J Cell Biol 163:715–721

    Article  Google Scholar 

  • Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC (2007) Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1. Structure 15:1392–1402

    Article  Google Scholar 

  • Strahl T, Huttner IG, Lusin JD, Osawa M, King D, Thorner J, Ames JB (2007) Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1). J Biol Chem 282:30949–30959

    Article  Google Scholar 

  • Tanaka T, Ames JB, Harvey TS, Stryer L, Ikura M (1995) Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state. Nature 376:444–447

    Article  ADS  Google Scholar 

  • Tzingounis AV, Kobayashi M, Takamatsu K, Nicoll RA (2007) Hippocalcin gates the calcium activation of the slow afterhyperpolarization in hippocampal pyramidal cells. Neuron 53:487–493

    Article  Google Scholar 

  • Vijay-Kumar S, Kumar VD (1999) Crystal structure of recombinant bovine neurocalcin. Nat Struct Biol 6:80–88

    Article  Google Scholar 

  • Villalobos C, Andrade R (2010) Visinin-like neuronal calcium sensor proteins regulate the slow calcium-activated afterhyperpolarizing current in the rat cerebral cortex. J Neurosci 30:14361–14365

    Article  Google Scholar 

  • Wishart DS, Sykes BD, Richards FM (1992) The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry 31:1647–1651

    Article  Google Scholar 

  • Zozulya S, Stryer L (1992) Calcium-myristoyl protein switch. Proc Natl Acad Sci USA 89:11569–11573

    Article  ADS  Google Scholar 

  • Zozulya S, Ladant D, Stryer L (1995) Expression and characterization of calcium-myristoyl switch proteins. Methods Enzymol 250:383–393

    Article  Google Scholar 

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Acknowledgments

We thank Jerry Dallas for technical support and help with NMR experiments. Work supported by NIH grants (EY012347) to J.B.A and (RR11973) to the UC Davis NMR facility.

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Correspondence to James B. Ames.

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Li, C., Ames, J.B. 1H, 13C, and 15N chemical shift assignments of neuronal calcium sensor protein, hippocalcin. Biomol NMR Assign 8, 63–66 (2014). https://doi.org/10.1007/s12104-012-9453-3

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  • DOI: https://doi.org/10.1007/s12104-012-9453-3

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