Abstract
Dictyostelium discoideum Formin C (ForC) plays an important role in the fruiting body formation during the multicellular stages of the slime mold. Formins are multidomain proteins that are known to regulate the actin cytoskeleton. Here, we report the assignments of the 1H, 15N, and 13C nuclei of the N-terminal activation domain (residues 1–100) of ForC. Chemical shifts have been deposited at the BioMagResBank under the BMRB accession number 17,029. The N-terminal region of the 131 kDa ForC protein is supposed to form a GTPase-binding domain required for activation of the formin.
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Acknowledgments
We thank Diana Ludwig and Barbara Stopschinski for help in protein expression and purification. Prof. Dr. Stephan Grzesiek from the Biozentrum of the University of Basel in Switzerland, we thank for providing the NMR infrastructure as well as for general support to S.A.D. during her professional time in Basel. This work was supported by grants from the Deutsche Forschungsgemeinschaft to S.A.D. (DA 1195/3-1) and M.G. (GE 976/4-2).
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Dames, S.A., Schönichen, A. & Geyer, M. 1H, 15N, and 13C assignments of the N-terminal activation domain of Dictyostelium discoideum Formin C. Biomol NMR Assign 5, 47–49 (2011). https://doi.org/10.1007/s12104-010-9264-3
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DOI: https://doi.org/10.1007/s12104-010-9264-3