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Human colorectal cancer derived-MSCs promote tumor cells escape from senescence via P53/P21 pathway

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Abstract

Purpose

The purpose of this study was to evaluate effect of MSCs on CRC cell.

Methods

in this study the MSC was isolated from CRC tissue, its effect on CRC cells was investigated in vivo and vitro, and the underlying mechanism was investigated.

Results

In this study we found that MSC-CM could promote colorectal cancer cells escape from senescence both in vitro and in vivo. Further research we demonstrated that MSC-CM acted in colorectal cancer cells senescence through P53/P21 pathway. Next we found that MSC-CM regulate P53 via posttranscription method.

Conclusion

Collectively, these results reveal that MSCs can help colorectal cancer cells defend against senescence through P53/P21 pathway, which may be a new strategy for colorectal cancer therapy.

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Abbreviations

CRC:

Colorectal cancer

MSC:

Mesenchymal stem cells

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Funding

This work was supported by the National Natural Sciences Foundation of China (no. 81372323 to Y.F.).

Author information

Authors and Affiliations

  1. Cancer Research Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China

    G. Li, R. Zhang, X. Zhang, S. Shao, F. Hu & Y. Feng

Authors
  1. G. Li
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  2. R. Zhang
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  3. X. Zhang
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  4. S. Shao
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  5. F. Hu
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  6. Y. Feng
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Contributions

GL contributed to the conception and design of the study; RZ, FH, GL and SS contributed to data collection; GL wrote the paper; All authors revised the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Y. Feng.

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Conflict of interest

The authors declare that they have no competing interests.

Consent for publication

The manuscript has been read and approved in the present form for submission by all named authors.

Ethics approval and consent to participate

This project got the permission of the patients and was approved by the ethics committee of The Tongji Hospital of Huazhong University of Science and Technology.

Human and animal rights statement

All procedures performed in the studies involving human participants were in accordance with 1964 Helsinki declaration and its later amendments or comparable ethical standards. Animal experiment were used under conditions approved by The Tongji Hospital of Huazhong University of Science and Technology.

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All participants provided informed consent prior to their participation.

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Li, G., Zhang, R., Zhang, X. et al. Human colorectal cancer derived-MSCs promote tumor cells escape from senescence via P53/P21 pathway. Clin Transl Oncol 22, 503–511 (2020). https://doi.org/10.1007/s12094-019-02152-5

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  • DOI: https://doi.org/10.1007/s12094-019-02152-5

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