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Targeted Metabolic and Genomic Profiling Reveals Parents for L-Dopa Breeding in Mucuna pruriens (L.) DC.

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Abstract

Integrating targeted metabolic profiling with genetic diversity estimates is vital for selecting genetic stocks for breeding as well as mapping a biochemical trait. In Mucuna pruriens (L.) DC., copious amount of L-Dopa in seeds is viewed both as a boon and bane. Our objective of this work was to assess in conjunction both L-Dopa and genomic diversity in a selected set of germplasm to elucidate their relationship and identify parents for L-Dopa breeding/mapping. The findings revealed good genetic as well as metabolic (L-Dopa) diversity among the studied accessions. However, we could not establish direct relationship between these two as the marker data measured using AFLP and ISSR were not correlated with the seed L-Dopa contents. Based on the consensus information from both the data sets, seven parental combinations have been suggested. These findings are expected to pave way for genetic improvement as well as genetic mapping of L-Dopa trait besides integration of molecular markers in M. pruriens breeding programs.

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Acknowledgments

Authors thank Department of Biotechnology (DBT), Govt. of India for the financial support; Sikkim University and Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore, India for the facilities; Dr. S A Ranade, National Botanical Research Institute (NBRI), Lucknow, India and Dr. G Ravikanth, Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore India for ISSR markers.

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Authors and Affiliations

  1. Department of Biotechnology, Sir M Visvesvaraya Institute of Technology, Bangalore, -562157, India

    M. Leelambika, S. Mahesh & M. Jaheer

  2. Department of Botany, Sikkim University, 6th Mile Samdur, P.O. Tadong, Gangtok, Sikkim, 737102, India

    P. K. Tripathi, P. Ranjith Kumar & N. Sathyanarayana

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  1. M. Leelambika
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  2. S. Mahesh
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  3. M. Jaheer
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  4. P. K. Tripathi
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  5. P. Ranjith Kumar
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  6. N. Sathyanarayana
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Correspondence to N. Sathyanarayana.

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All the funding agencies and support levied are acknowledged

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Communicated by: Paulo Arruda

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Leelambika, M., Mahesh, S., Jaheer, M. et al. Targeted Metabolic and Genomic Profiling Reveals Parents for L-Dopa Breeding in Mucuna pruriens (L.) DC.. Tropical Plant Biol. 9, 239–251 (2016). https://doi.org/10.1007/s12042-016-9174-3

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