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A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria

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Abstract

Peanut (Arachis hypogaea) is amongst the most important legume crops in the world. One of its main yield constraints is the root-knot nematode Meloidogyne arenaria. A number of wild Arachis species, including A. stenosperma, are resistant to nematodes, and are a potential source of new resistance alleles for cultivated peanut. Using in silico subtraction of ESTs and macroarray analysis, we identified genes differentially expressed in A. stenosperma roots during its resistance response to M. arenaria. The three most differentially expressed genes [Auxin Repressed Protein (AsARP), Cytokinin Oxidase (AsCKX) and Metallothionein Type 2 (AsMET2)] were further analyzed using northern-blot and showed distinct expression profiles in the resistant A. stenosperma and susceptible A. hypogaea, both after, and sometimes even before, challenge with nematodes. Of the three most differentially expressed genes, AsARP and AsCKX are potentially involved in plant hormonal balance, and AsMET2 may be related to the reactive oxygen reaction triggered by the hypersensitive response (HR).

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Acknowledgements

The authors gratefully acknowledge The Challenge Program Generation, Tropical Legume Improvement (TL1), CNPq, FAP-DF and host institutions for funding this research. The authors also wish to thank Dr. Regina D.G. Carneiro (Embrapa Recursos Genéticos e Biotecnologia, Brazil) for providing nematodes.

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Authors and Affiliations

  1. EMBRAPA Recursos Genéticos e Biotecnologia, Parque Estação Biológica, CP 02372, Final W5 Norte, Brasília, DF, Brazil

    Patricia Messenberg Guimarães, Ana Cristina Miranda Brasileiro, Ana Claudia Guerra de Araújo, Soraya Cristina Macedo Leal-Bertioli, Felipe Rodrigues da Silva, Carolina Vianna Morgante & Simone da Graça Ribeiro

  2. Universidade de Brasília, Campus I, Brasília, DF, Brazil

    Karina Proite, Aline Pic-Taylor & David John Bertioli

  3. Universidade Católica de Brasília, Campus II, 916 Norte, Brasília, DF, Brazil

    David John Bertioli

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  1. Patricia Messenberg Guimarães
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  2. Ana Cristina Miranda Brasileiro
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  3. Karina Proite
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  4. Ana Claudia Guerra de Araújo
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  8. Carolina Vianna Morgante
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  9. Simone da Graça Ribeiro
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  10. David John Bertioli
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Correspondence to Patricia Messenberg Guimarães.

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Communicated by: John Manners

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Table S-1

List of 75 genes selected for macroarray analysis from A. stenosperma RM and RN cDNA libraries by the following criteria: (A) Putative constitutively expressed clones; (B) Clones differentially expressed (P ≤ 0.05); (C) Clones exclusive to RM library and related to plant-pathogen interaction; (D) Clones responsive to plant stress. (XLSX 19 kb)

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Guimarães, P.M., Brasileiro, A.C.M., Proite, K. et al. A Study of Gene Expression in the Nematode Resistant Wild Peanut Relative, Arachis stenosperma, in Response to Challenge with Meloidogyne arenaria . Tropical Plant Biol. 3, 183–192 (2010). https://doi.org/10.1007/s12042-010-9056-z

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