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Cloning and expression analysis of a new anther-specific gene CaMF4 in Capsicum annuum

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Abstract

Our previous study on the genic male sterile–fertile line 114AB of Capsicum annuum indicated a diversity of differentially expressed cDNA fragments in fertile and sterile lines. In this study, a transcript-derived fragment (TDF), male fertile 4 (CaMF4) was chosen for further investigation to observe that this specific fragment accumulates in the flower buds of the fertile line. The full genomic DNA sequence of CaMF4 was 894 bp in length, containing two exons and one intron, and the complete coding sequence encoded a putative 11.53 kDa protein of 109 amino acids. The derived protein of CaMF4 shared similarity with the members of PGPS/D3 protein family. The expression of CaMF4 was detected in both the flower buds at stage 8 and open flowers of the male fertile line. In contrast to this observation, expression of CaMF4 was not detected in any organs of the male sterile line. Further analysis revealed that CaMF4 was expressed particularly in anthers of the fertile line. Our results suggest that CaMF4 is an anther-specific gene and might be indispensable for anther or pollen development in C. annuum.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31572124), the Specialized Research fund for the Doctoral Program of Higher Education of China (no. 20134404110017) and the Principal Foundation of South China Agricultural University (2013).

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Authors and Affiliations

  1. Department of Biology, Taiyuan Normal University, Taiyuan, 030012, Shanxi Province, People’s Republic of China

    XUEFENG HAO & JIANJUN LEI

  2. College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong Province, People’s Republic of China

    XUEFENG HAO, CHANGMING CHEN, GUOJU CHEN, BIHAO CAO & JIANJUN LEI

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  2. CHANGMING CHEN
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Correspondence to JIANJUN LEI.

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Corresponding editor: Rajiva Raman

Hao X., Chen C., Chen G., Cao B. and Lei J. 2017 Cloning and expression analysis of a new anther-specific gene CaMF4 in Capsicum annuum. J. Genet. 96, xx–xx

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HAO, X., CHEN, C., CHEN, G. et al. Cloning and expression analysis of a new anther-specific gene CaMF4 in Capsicum annuum . J Genet 96, 25–31 (2017). https://doi.org/10.1007/s12041-016-0735-x

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  • DOI: https://doi.org/10.1007/s12041-016-0735-x

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