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Association of interleukin-10 (A1082G) gene polymorphism with oral squamous cell carcinoma in north Indian population

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Abstract

The functional polymorphism A1082G in the gene (IL10) for interleukin-10 associated with risk of oral squamous cell carcinoma (OSCC). The present case–control study was to evaluate the possible association between IL10 A1082G gene and OSCC in north Indian population. Analysis of IL10 A1082G genotype in 232 OSCC cases and 221 healthy controls of comparable age, gender, smokers, tobacco chewing and alcohol consumption. IL10 A1082G status in cases and controls were evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The frequencies of IL10 A1082G polymorphism AA, AG, GG genotypes were 29.74, 68.10 and 2.15% in OSCC cases and 57.46, 42.08 and 0.45% in healthy controls. The average frequency of G mutant allele was 36.20% in OSCC cases compared with 21.50% among the controls and this allele was associated with increased risk for OSCC cases. Heterozygous AG genotype was found statistically significant in OSCC cases than in controls (OR = 1.6, 95% CI = 1.1–2.2, P = 0.003), whereas homozygous mutant GG genotype was not found significant (OR = 4.7, 95% CI = 0.55–41.1, P = 0.2). Moreover, we found that G allele was significant in OSCC cases of tobacco chewing. The frequency of IL10 A1082G polymorphism G allele and AG genotype is associated with OSCC cases as compared with controls; this may be due to smoking and tobacco chewing. Our findings showed that in IL10 A1082G gene polymorphism AG genotype and G allele may participate in the progression of OSCC.

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Acknowledgements

This study was supported by Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India.

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Authors and Affiliations

  1. Molecular Cell Biology Lab, Department of Biochemistry, King George’s Medical University, Lucknow, 226 003, India

    SYED RIZWAN HUSSAIN, MOHAMMAD KALEEM AHMAD, ABBAS ALI MAHDI, HENA NAQVI & MOHAMMAD WASEEM AHMAD

  2. Oral Pathology and Microbiology, King George’s Medical University, Lucknow, 226 003, India

    SAURABH SRIVASTAVA, KUMUD NIGAM & SHALINI GUPTA

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  1. SYED RIZWAN HUSSAIN
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Correspondence to SHALINI GUPTA.

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[Hussain S. R., Ahmad M. K., Mahdi A. A., Naqvi H., Ahmad M. W., Srivastava S., Nigam K. and Gupta S. 2016 Association of interleukin-10 (A1082G) gene polymorphism with oral squamous cell carcinoma in north Indian population. J. Genet. 95, xx–xx]

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HUSSAIN, S.R., AHMAD, M.K., MAHDI, A.A. et al. Association of interleukin-10 (A1082G) gene polymorphism with oral squamous cell carcinoma in north Indian population. J Genet 95, 249–255 (2016). https://doi.org/10.1007/s12041-016-0626-1

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