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Autophagy requires Tip20 in Saccharomyces cerevisiae

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Abstract

Autophagy is a highly conserved intracellular degradation pathway in eukaryotic cells that responds to environmental changes. Genetic analyses have shown that more than 40 autophagy-related genes (ATG) are directly involved in this process in fungi. In addition to Atg proteins, most vesicle transport regulators are also essential for each step of autophagy. The present study showed that one Endoplasmic Reticulum protein in Saccharomyces cerevisiae, Tip20, which controls Golgi-to-ER retrograde transport, was also required for starvation-induced autophagy under high temperature stress. In tip20 conditional mutant yeast, the transport of Atg8 was impaired during starvation, resulting in multiple Atg8 puncta dispersed outside the vacuole that could not be transported to the pre-autophagosomal structure/phagophore assembly site (PAS). Several Atg8 puncta were trapped in ER exit sites (ERES). Moreover, the GFP-Atg8 protease protection assay indicated that Tip20 functions before autophagosome closure. Furthermore, genetic studies showed that Tip20 functions downstream of Atg5 and upstream of Atg1, Atg9 and Atg14 in the autophagy pathway. The present data show that Tip20, as a vesicle transport regulator, has novel roles in autophagy.

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Abbreviations

Atg:

autophagy-related gene

ER:

endoplasmic reticulum

ERES:

endoplasmic reticulum exit sites

GFP:

green fluorescent protein

PAS:

pre-autophagosomal structure/phagophore assembly site

PE:

phosphatidylethanolamine

RFP:

red fluorescent protein.

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Acknowledgements

We are grateful to H. Schmitt for providing WT, tip20-5 and dsl1-22 strains, J Nunnaria for providing DsRed-HDEL-305 plasmids, and Q Jin for providing pFA6a-tdTomato-KanMx plasmid. This work was supported by grants from the Natural Science Foundation of China (31301173 to SZ and 31101428 to LC) and Shandong Agricultural University Talent Introduction Funding (20171226 to HD).

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Authors and Affiliations

  1. Department of Plant Pathology, Shandong Agricultural University, Tai’an, 271018, China

    Lei Chen, Chunling Zhang, Yuancun Liang, Aixin Liu, Hansong Dong & Shenshen Zou

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  1. Lei Chen
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  2. Chunling Zhang
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  3. Yuancun Liang
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  4. Aixin Liu
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  5. Hansong Dong
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  6. Shenshen Zou
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Correspondence to Shenshen Zou.

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Corresponding editor: Sudha Bhattacharya

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Chen, L., Zhang, C., Liang, Y. et al. Autophagy requires Tip20 in Saccharomyces cerevisiae. J Biosci 44, 17 (2019). https://doi.org/10.1007/s12038-018-9839-1

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