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PRKCH 1425G/A Polymorphism Predicts Recurrence of Ischemic Stroke in a Chinese Population

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Abstract

A recent genome-wide association study (GWAS) identified a nonsynonymous SNP (1425G/A) in PRKCH which was associated with increased risk of ischemic stroke. The purpose of this study was to examine whether this functional polymorphism is associated with stroke onset and prognosis in a Chinese population. We genotyped PRKCH 1425G/A using Improved Multiple Ligase Detection Reaction in 919 patients with ischemic stroke. Analyses of genotype association with onset and prognosis outcomes were assessed by the Kaplan-Meier method, the log-rank test, and the Cox proportional hazards models. PRKCH 1425G/A was not associated with age of stroke onset (P = 0.323). However, this functional polymorphism was significantly associated with risk of stroke recurrence in recessive models (hazard ratio [HR] = 2.23; 95 % confidential interval [CI], 1.06 to 4.68; P = 0.014), and this effect was more predominant among smokers (HR = 3.67; 95 % CI, 1.47–9.18; P = 0.005). Moreover, the variant genotypes of PRKCH 1425G/A are an independent prognostic factor for ischemic stroke in the final multivariate Cox regression model. Our findings show that PRKCH 1425G/A may be a useful biomarker for predicting the recurrence of ischemic stroke.

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Abbreviations

HR:

Hazard ratio

CI:

Confidence interval

GWAS:

Genome-wide association study

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31200938, 81220108008); Natural Science Foundation of Jiangsu Province (BK2011021); and Natural Science Foundation of Jinling Hospital (2012009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors thank Benjamin L. Kidder (Systems Biology Center, National Heart, Lung and Blood Institue, National Institutes of Health, Bethesda, MD 20892, USA) for critical review and language editing of the manuscript.

Conflict of Interests

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Neurology, Jinling Hospital, Nanjing University School of Medicine, 305# East Zhongshan Road, Nanjing, 210002, Jiangsu Province, China

    Zhizhong Zhang, Gelin Xu, Wusheng Zhu, Liping Cao & Xinfeng Liu

  2. Melbourne Brain Centre, Royal Melbourne Hospital, University of Melbourne, Melbourne, Australia

    Bernard Yan

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  1. Zhizhong Zhang
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  2. Gelin Xu
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  3. Wusheng Zhu
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  4. Liping Cao
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  5. Bernard Yan
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Correspondence to Xinfeng Liu.

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Zhang, Z., Xu, G., Zhu, W. et al. PRKCH 1425G/A Polymorphism Predicts Recurrence of Ischemic Stroke in a Chinese Population. Mol Neurobiol 52, 1648–1653 (2015). https://doi.org/10.1007/s12035-014-8964-6

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