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Preparation and Characterization of Magnetite-Polyvinyl Alcohol Hybrid Nanoparticles (As-PVA-MNPs) Using Acanthophora spicifera Marine Algae Extract for Enhanced Antimicrobial Activity Against Pathogenic Microorganisms

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Abstract

The present study focused on preparing and characterizing magnetite-polyvinyl alcohol (PVA) hybrid nanoparticles using Acanthophora spicifera marine algae extract as a reducing agent. Various analytical techniques, including UV–Visible spectrometry, Fourier-transform infrared (FTIR) analysis, energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analysis, were used to characterize the nanoparticles. The results showed the successful synthesis of nanoparticles with a characteristic color change and absorption peak at 400 nm in UV–Visible spectrometry. FTIR analysis indicated an interaction between the carboxyl group and magnetite-polyvinyl alcohol hybrid ions. SEM analysis revealed spherical nanoparticles with sizes ranging from 20 to 100 nm. EDX analysis confirmed the presence of strong magnetite peaks in Acanthophora spicifera, validating successful preparation. XRD analysis indicated the crystalline nature of the nanoparticles. Furthermore, the antimicrobial potential of As-PVA-MNPs was evaluated, demonstrating a significant zone of inhibition against tested bacterial and fungal samples at a concentration of 100 µg. These findings suggest the promising antimicrobial activity of the synthesized nanoparticles for potential applications in combating pathogenic microorganisms.

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Data Availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors extend their appreciation to the Researchers supporting project number (RSP2023R316) King Saud University, Riyadh, Saudi Arabia National Center for Inter-University Research Facilities, Seoul National University 2020R1G1A1012069.

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Authors and Affiliations

  1. Department of Biotechnology, PSGR krishnammal College for Women, Coimbatore, Tamil Nadu, 641004, India

    D. S. Ranjith Santhosh Kumar

  2. School of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore, Tamil Nadu, 641014, India

    S. Hari Puthiran & P. Senthilkumar

  3. Department of Biotechnology, KIT-Kalaignarkarunanidhi Institute of Technology, Coimbatore, Tamil Nadu, 641402, India

    Gayathri Devi Selvaraju

  4. School of Bioscience and Technology, VIT- Vellore Institute of Technology, Vellore, Tamil Nadu, 632014, India

    Paul A. Matthew

  5. PG and Research Department of Biotechnology & Bioinformatics, Holy Cross College, Tiruchirappalli, Tamil Nadu, 620002, India

    Sowmya Kuppusamy

  6. Faculty of Pharmaceutical Sciences, UCSI University, 56000, Cheras, Kuala Lumpur, Malaysia

    Ravishankar Ram Mani

  7. Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, 1155, Riyadh, Saudi Arabia

    Ashraf Atef Hatamleh & Munirah Abdullah AI- Dosary

  8. Department of Medical Biotechnology and Integrative Physiology, Institute of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nadu, 602105, India

    Soon Woong Chang & Balasubramani Ravindran

  9. Department of Environmental Energy and Engineering, Kyonggi University, Yeongtong-Gu, Suwon, Gyeonggi-Do, 16227, Republic of Korea

    Balasubramani Ravindran

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Contributions

Conceptualization, RSKDS and SP.; methodology and validation, HS; formal analysis, GS; investigation, RKDS; data curation, GD; writing and original draft preparation, PAM; writing, reviewing, and editing of the manuscript, HS, RRM, AAH, MAAD, SWC, and BR; visualization, SP; supervision and project administration, RSDS.

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Correspondence to D. S. Ranjith Santhosh Kumar or Balasubramani Ravindran.

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Kumar, D.S.R.S., Puthiran, S.H., Selvaraju, G.D. et al. Preparation and Characterization of Magnetite-Polyvinyl Alcohol Hybrid Nanoparticles (As-PVA-MNPs) Using Acanthophora spicifera Marine Algae Extract for Enhanced Antimicrobial Activity Against Pathogenic Microorganisms. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00903-y

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