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Hsa_circ_0001741 Suppresses Ovarian Cancer Cell Proliferations Through Adsorption of miR-188-5p and Promotion of FOXN2 Expression

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Abstract

Ovarian cancer (OC) is among several general malignant gynecological cancers associated with high mortality rates on a global scale. Earlier investigations have revealed a critical role of circular RNAs (circRNAs) in OC development, which is a new class of endogenous non-coding RNA (ncRNA) that reported to mediate progression of diverse tumor types. At present, the precise involvement of circRNAs and associated regulatory mechanisms in OC remain unknown. In this study, hsa_circ_0001741 expression patterns in OC cells and tissues were tested. The underlying regulatory pathways and targets were further explored with the aid of bioinformatics, luciferase reporter, 5-ethynyl-2′-deoxyuridine (EdU) and cell counting kit-8 (CCK-8) analyses. Further investigation of the hsa_circ_0001741 effects on tumor growth in vivo revealed abnormal circRNA expression in OC. hsa_circ_0001741 expression reduced in OC cells and tissues, indicative of activity in OC progression. hsa_circ_0001741 upregulation resulted in OC proliferation inhibitions. The luciferase reporter outputs verified miR-188-5p and FOXN2 as hsa_circ_0001741 downstream targets. FOXN2 silencing or miR-188-5p upregulations reversed inhibitory effects regarding hsa_circ_0001741 on OC cell proliferation. Therefore our data suggested that hsa_circ_0001741 upregulation inhibited proliferation of OC through modulatory effects on miR-188-5p/FOXN2 signaling.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, The Xiang’an Affiliated Hospital of Xiamen University, No 2000, Xiang’an East Road, Xindian Town, Xiang’an District, 361102, Xiamen, Fujian Province, China

    Hong Wang, Jing Lin, Yanan Dong & Yangyang Wang

  2. Department of Obstetrics and Gynecology, The Affiliated Hospital of Beihua University, Jilin, China

    Caijuan Liang

  3. The pharmacy college of Xiamen university, Xiamen, China

    Lin Xia

Authors
  1. Hong Wang
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  2. Caijuan Liang
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  3. Jing Lin
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  4. Yanan Dong
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  5. Yangyang Wang
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  6. Lin Xia
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Contributions

Hong Wang contributed to the study conception and design. All authors collected the data and performed the data analysis. All authors contributed to the interpretation of the data and the completion of figures and tables. All authors contributed to the drafting of the article and final approval of the submitted version.

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Correspondence to Hong Wang.

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Ethics Committee in Xiang’an Affiliated Hospital of Xiamen University (XMULAC20220151) approved the study. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Participants or their relatives were informed of the investigation and consent forms were signed prior to their inclusion in the trial.

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Wang, H., Liang, C., Lin, J. et al. Hsa_circ_0001741 Suppresses Ovarian Cancer Cell Proliferations Through Adsorption of miR-188-5p and Promotion of FOXN2 Expression. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00773-4

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  • DOI: https://doi.org/10.1007/s12033-023-00773-4

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