Abstract
In this study, BALB/c mice with Ehrlich solid tumors were used to examine the effect of Achillea millefolium L. (AM) extract on the Ehrlich ascites tumor (EAT) model, which is one of the experimental cancer models. Also known as yarrow and plant, AM has antioxidant, anti-inflammatory, antibacterial and antitumor properties. In our study, 57 male BALB/c type mice, 8–10 weeks old, weighing 25–30 g, were used. Mice were divided into two groups. Ehrlich Solid Tumor group: Negative Control Group (ENC), Positive Control Group (EPC), and Treatment Group (TG) (TNCAM-200 mg/kg, TPCAM-400 mg/kg). EPC and TG were given to EAT cells. Each EAT contained 1 × 106 (will be 6 out of 10: so:000000) EAT cells, 0.1 ml of phosphate-buffered saline (PBS) was administered subcutaneously (s.c.) to the nape of mice. Then It was awaited for solid tumor formation. AM extract was administered intraperitoneally (i.p.) to TG for 17 days to mice. AM extract was found to have a curative effect on areas of inflammation, bleeding, and necrosis in treatment groups treated with AM extract alone. The treatment groups showed nearly normal histological results compared to the positive control group. According to the results, the TPCAM-400 mg/kg group had a more significant histological impact than the TNCAM-200 mg/kg group. In terms of tumor growth, tumor length, tumor volume, and tumor weight, AM extract did not show significant effects. However, in the light of histological findings, promising results of AM were observed in mice in which Ehrlich Solid Tumor was formed.
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Acknowledgements
This research was produced from Gökçe BAĞCI UZUN’s Ph.D. thesis named “Investıgatıon Of The Antıtumoral Effect Of On Ehrlıch Solıd Tumor-Formed Mıce Of Yarrow (Achilea Milllefolium)” is created. The financial source of this research was funded by Erciyes University Scientific Research Projects Coordination Unit.
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This study was supported by Erciyes University Scientific Research Projects Unit with a project numbered TDK-2018–8188.
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GBU and MN: designed the study, analyzed and interpreted the data, wrote and edited the manuscript. AA: designed the study, collected and analyzed the data, wrote and revised the manuscript. AHY: performed histopathological examination. GŞK: yarrow prepared her extract. SU and ÖA: they participated in the experimental study. GBU: was accepted as the editorial author, and article control was provided by all authors and it was confirmed to be a collaborative work.
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This study was initiated with the decision of Erciyes University Experimental Animals Local Ethics Committee no. 17/071 dated 16.08.2017, and the ethics committee decisions were adhered to during the study.
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Oral presentation made; Bağcı Uzun G, Nisari M, Hanım Yay A, Karatoprak Şeker G, Al Ö, Uçar S. Civan Perçemi Investıgatıon Of The Antıtumoral Effect Of On Ehrlich Solıd Tumor-Formed Mıce Of Yarrow(Achillea Milllefolium). 2nd International Congress on Sports,Anthropology, Nutrition, Anatomy and Radiology 20–23 July 2020, Nevşehir, Turkey.
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Bağcı Uzun, G., Nisari, M., Hanım Yay, A. et al. Investigating the anti-tumoral effect of yarrow (Achillea milllefolium) on the mice in which ehrlich solid tumor is created. Med Oncol 40, 42 (2023). https://doi.org/10.1007/s12032-022-01917-3
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DOI: https://doi.org/10.1007/s12032-022-01917-3