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MicroRNA-204-5p inhibits gastric cancer cell proliferation by downregulating USP47 and RAB22A

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Abstract

MicroRNAs (miRNAs) are a type of small noncoding RNAs that are strongly implicated in carcinogenesis. However, the potential diagnostic, prognostic and therapeutic roles of the majority of miRNAs in the pathological processes of tumorigenesis remain largely unknown. Our and others’ data revealed that miR-204-5p was significantly downregulated in gastrointestinal tumor tissues compared with adjacent noncancerous tissues. The downregulation of miR-204-5p was confirmed in our gastric cancer (GC) cohort, and we showed that ectopic expression of miR-204-5p inhibited, whereas silencing miR-204-5p expression promoted GC cell proliferation in vitro. Subsequent mechanistic investigations identified that USP47 and RAB22A are direct functional targets of miR-204-5p in GC. Silencing the expression of USP47 and RAB22A using siRNA phenocopied the proliferation-inhibiting function of miR-204-5p in GC cells. Our results uncovered that miR-204-5p acts as a tumor suppressor in GC through inhibiting USP47 and RAB22A.

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Acknowledgments

This study was partially supported by grants from the National Natural 39 Science Foundation of China (Nos. 81000867, 81272299, and 81301784) and Medical Key Professionals Program of Jiangsu Province (RC2011031).

Conflict of interest

The authors report no conflict of interests. The authors alone are responsible for the content and writing of the paper.

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Authors and Affiliations

  1. Oncology Institute, The Fourth Affiliated Hospital of Soochow University, The Fourth People’s Hospital of Wuxi, Wuxi, 214062, Jiangsu Province, China

    Binbin Zhang & Zhaohui Huang

  2. Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, 214062, Jiangsu, China

    Yuan Yin, Yaling Hu, Jiwei Zhang, Zehua Bian, Mingxu Song, Dong Hua & Zhaohui Huang

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  1. Binbin Zhang
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Correspondence to Zhaohui Huang.

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Zhang, B., Yin, Y., Hu, Y. et al. MicroRNA-204-5p inhibits gastric cancer cell proliferation by downregulating USP47 and RAB22A. Med Oncol 32, 331 (2015). https://doi.org/10.1007/s12032-014-0331-y

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