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Association of MicroRNAs with the Clinicopathologic Characteristics of Ependymoma

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Abstract

The current management of ependymoma is wrought with limitations. Molecular classification is a promising development. MicroRNA (miRNA) deregulation is associated with human cancer and may be a means of molecular classification. The aim of our study is to investigate the association of miRNA expression with the clinicopathologic characteristics of ependymoma. Twenty-two samples were clinically annotated. Histologic features were reassessed and the expression of Ki-67, cyclin D1, and nestin was examined. The expression of 84 stem cell-related miRNAs was profiled. The ΔΔCT method and a Student’s t test were used to compute fold changes and P values, respectively. Our analysis revealed 24 statistically significant associations. We identified seven site-specific miRNAs. The pattern of expression was variable in each anatomic site. In addition, we identified six candidate recurrence biomarkers, all of which were overexpressed in recurrent cases. All three grade-related miRNAs were underexpressed in anaplastic samples. Two miRNAs each were underexpressed in samples immunoreactive to Ki-67 and cyclin D1. No miRNAs were differentially expressed between nestin-negative and nestin-positive samples. In conclusion, molecular alterations in ependymoma involve miRNAs. In our report, we review the level of evidence for the biomarker candidacy of identified miRNAs. Confirmatory studies are necessary to establish robust biomarkers for the clinical management of ependymoma. Proteins regulated by differentially expressed miRNAs are additional candidate biomarkers and may offer targets for novel therapeutic interventions.

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Funding

This work was supported by the King Abdullah II Fund for Development and the Scientific Research Department of the King Abdullah II Design and Development Bureau (Grant Numbers 15, 16; 2015).

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Author notes

  1. Justin Z. Amarin and Haya H. Suradi contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology and Biochemistry, School of Medicine, The University of Jordan, Amman, Jordan

    Mamoun Ahram & Randa M. Bawadi

  2. School of Medicine, The University of Jordan, Amman, Jordan

    Justin Z. Amarin, Haya H. Suradi, Sultan S. Abdelhamid & Mona M. Makhamreh

  3. Department of Pathology and Laboratory Medicine, King Hussein Cancer Center, Amman, Jordan

    Maysa Al-Hussaini

Authors
  1. Mamoun Ahram
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  2. Justin Z. Amarin
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  3. Haya H. Suradi
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  4. Sultan S. Abdelhamid
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  5. Mona M. Makhamreh
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  6. Randa M. Bawadi
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  7. Maysa Al-Hussaini
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Correspondence to Mamoun Ahram.

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The study protocol was approved by the Institutional Review Board of the King Hussein Cancer Center.

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The authors declare that they have no conflicts of interest.

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Ahram, M., Amarin, J.Z., Suradi, H.H. et al. Association of MicroRNAs with the Clinicopathologic Characteristics of Ependymoma. J Mol Neurosci 66, 307–313 (2018). https://doi.org/10.1007/s12031-018-1178-z

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  • DOI: https://doi.org/10.1007/s12031-018-1178-z

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