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Valproic Acid Promotes Survival of Facial Motor Neurons in Adult Rats After Facial Nerve Transection: a Pilot Study

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Abstract

Valproic acid (VPA), a medication primarily used to treat epilepsy and bipolar disorder, has been applied to the repair of central and peripheral nervous system injury. The present study investigated the effect of VPA on functional recovery, survival of facial motor neurons (FMNs), and expression of proteins in rats after facial nerve trunk transection by functional measurement, Nissl staining, TUNEL, immunofluorescence, and Western blot. Following facial nerve injury, all rats in group VPA showed a better functional recovery, which was significant at the given time, compared with group NS. The Nissl staining results demonstrated that the number of FMNs survival in group VPA was higher than that in group normal saline (NS). TUNEL staining showed that axonal injury of facial nerve could lead to neuronal apoptosis of FMNs. But treatment of VPA significantly reduced cell apoptosis by decreasing the expression of Bax protein and increased neuronal survival by upregulating the level of brain-derived neurotrophic factor (BDNF) and growth associated protein-43 (GAP-43) expression in injured FMNs compared with group NS. Overall, our findings suggest that VPA may advance functional recovery, reduce lesion-induced apoptosis, and promote neuron survival after facial nerve transection in rats. This study provides an experimental evidence for better understanding the mechanism of injury and repair of peripheral facial paralysis.

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Funding

This work was supported by grants from the National 973 Basic Research Program of China (2014CB541703) and grants from the National Natural Science Foundation of China (81670932).

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Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China

    Lili Zhang, Zhaomin Fan, Yuechen Han, Lei Xu & Haibo Wang

  2. Department of Otolaryngology-Head and Neck Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264199, China

    Lili Zhang

  3. Shandong Provincial Key Laboratory of Otology, Jinan, 250022, China

    Wenwen Liu, Xiaohui Bai, Meijuan Zhou, Jianfeng Li & Haibo Wang

  4. Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250022, China

    Jianfeng Li & Haibo Wang

Authors
  1. Lili Zhang
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  2. Zhaomin Fan
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  3. Yuechen Han
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  4. Lei Xu
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  5. Wenwen Liu
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  6. Xiaohui Bai
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  7. Meijuan Zhou
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  8. Jianfeng Li
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  9. Haibo Wang
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Correspondence to Haibo Wang.

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Zhang, L., Fan, Z., Han, Y. et al. Valproic Acid Promotes Survival of Facial Motor Neurons in Adult Rats After Facial Nerve Transection: a Pilot Study. J Mol Neurosci 64, 512–522 (2018). https://doi.org/10.1007/s12031-018-1051-0

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  • DOI: https://doi.org/10.1007/s12031-018-1051-0

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