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Up-Regulation of NF45 Correlates with Schwann Cell Proliferation After Sciatic Nerve Crush

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Abstract

Nuclear factor (NF)45 (also known as interleukin enhancer-binding factor (ILF)2), is a transcription factor that interacts with NF90 to regulate gene expression. It has long been implicated in the regulation of cell proliferation. However, the role of NF45 in the process of peripheral nervous system regeneration after injury remains poorly understood. Herein, we investigated the spatiotemporal expression of NF45 in a rat sciatic nerve crush model. We detected the up-regulated expression of NF45 in Schwann cell after sciatic nerve crush. What’s more, the expression of the cell proliferation marker proliferating cell nuclear antigen (PCNA) exhibited a similar tendency with that of NF45. In cell cultures, we observed increased expression of NF45 during the process of TNF-α-induced Schwann cell proliferation, whereas the protein level of p21 was down-regulated. Interference of NF45 led to enhanced expression of p21 and also impaired proliferation of Schwan cells. Taken together, our data implicated that NF45 was up-regulated in the sciatic nerve after crush, which was associated with proliferation of Schwann cell.

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Abbreviations

SNC:

Sciatic nerve crush

CNS:

Central nervous system

PNS:

Peripheral nervous system

PNI:

Peripheral nerve injury

PCNA:

Proliferating cell nuclear antigen

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81171140,no. 81471258, no.31300902), the Colleges and Universities in Natural Science Research Project of Jiangsu Province (13KJB310009), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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All the authors of this study declare no conflict of interest.

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Correspondence to Yi Zhang.

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Youhua Wang and Shiran Zhou contributed equally to this work.

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Wang, Y., Zhou, S., Xu, H. et al. Up-Regulation of NF45 Correlates with Schwann Cell Proliferation After Sciatic Nerve Crush. J Mol Neurosci 56, 216–227 (2015). https://doi.org/10.1007/s12031-014-0484-3

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  • DOI: https://doi.org/10.1007/s12031-014-0484-3

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