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Changes of Peroxisome Proliferator-Activated Receptor-γ on Crushed Rat Sciatic Nerves and Differentiated Primary Schwann Cells

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Abstract

Peroxisome proliferator-activated receptor-γ (PPAR-γ) has been found to play an essential role in cell proliferation, but whether it was involved in Schwann cells differentiation has never been studied. We have found in sciatic nerve injury that expression of PPAR-γ decreases mainly in Schwann cells, and it was also increased in differentiated Schwann cells. Further, activated PPAR-γ by the endogenous ligand 15 d-PGJ2 increased expressions of PPAR-γ level and Schwann cell differentiation, and this effect may be protected by its antagonist GDW9662. These results indicate that PPAR-γ could promote Schwann cell differentiation, which plays an important role in peripheral nerve injury and regeneration.

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Abbreviations

SCs:

Schwann cells

c-AMP:

3′–5′-Cyclic adenosine monophosphate

PPAR-γ:

Peroxisome proliferator-activated receptor-γ

P0:

Myelin protein zero

15 d-PGJ2 :

15-Deoxy-Δ12,14-prostaglandin J2

CNS:

Central nervous system

PNS:

Peripheral nervous system

RT–PCR:

Reverse transcription–polymerase chain reaction

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81171743), the Natural Science Foundation of Jiangsu Province (No. BK2009161 and No. BK2010169), the Natural Science Foundation of Jiangsu Colleges and Universities Grant (09KJD310005), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Orthopaedics, the First Affiliated Hospital, Medical College, Soochow University, Suzhou, Jiangsu, 215123, People’s Republic of China

    Yi Cao, Zhengming Zhou & Youhua Wang

  2. Department of Immunology, Medical College, Nantong University, Nantong, 226001, People’s Republic of China

    Qiuhong Wang, Yonghua Liu & Yuhong Ji

  3. Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, People’s Republic of China

    Fan Liu

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  1. Yi Cao
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  2. Qiuhong Wang
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  3. Zhengming Zhou
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  4. Youhua Wang
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  5. Yonghua Liu
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  6. Yuhong Ji
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  7. Fan Liu
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Correspondence to Fan Liu.

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Yi Cao and Qiuhong Wang contributed equally to this study.

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Cao, Y., Wang, Q., Zhou, Z. et al. Changes of Peroxisome Proliferator-Activated Receptor-γ on Crushed Rat Sciatic Nerves and Differentiated Primary Schwann Cells. J Mol Neurosci 47, 380–388 (2012). https://doi.org/10.1007/s12031-011-9662-8

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  • DOI: https://doi.org/10.1007/s12031-011-9662-8

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