Abstract
We investigated whether activity-dependent neuroprotective protein (ADNP) could autoregulate its own expression. Both the endogenous ADNP gene and reporter gene constructs were analysed in response to overexpression of ADNP, supplied either as wild-type ADNP or a mutant form lacking the NAP motif, a motif which has neuroprotective properties. Overexpression of these two forms of ADNP resulted in both decreased endogenous ADNP expression and repressed ADNP promoter-directed reporter gene activity. Chromatin immunoprecipitation demonstrated the ability of ADNP to bind to its own promoter which is consistent with its action as a repressor of both promoter-supported and endogenous ADNP expression.
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Abbreviations
- PCR:
-
Polymerase chain reaction
- bp:
-
Base pair
- m:
-
Minutes
- s:
-
Seconds
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Acknowledgements
Funding from the BBSRC (ASV, KH, VJB and JPQ) and a studentship from the University of Taibah, Kingdom of Saudi Arabia (MSA) supported this work. We thank DNA Sequencing & Services (MRCPPU, College of Life Sciences, University of Dundee, Scotland, www.dnaseq.co.uk) for DNA sequencing and Dr. Fahad Ali for helpful discussions during this work.
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Moutasem S. Aboonq and Sylvia A. Vasiliou contributed equally to this work
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Aboonq, M.S., Vasiliou, S.A., Haddley, K. et al. Activity-Dependent Neuroprotective Protein Modulates Its Own Gene Expression. J Mol Neurosci 46, 33–39 (2012). https://doi.org/10.1007/s12031-011-9562-y
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DOI: https://doi.org/10.1007/s12031-011-9562-y