Abstract
Background
Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. Pathophysiological processes following initial insult are complex and not fully understood. Ionized calcium (Ca++) is an essential cofactor in the coagulation cascade and platelet aggregation, and hypocalcemia may contribute to the progression of intracranial bleeding. On the other hand, Ca++ is an important mediator of cell damage after TBI and cellular hypocalcemia may have a neuroprotective effect after brain injury. We hypothesized that early hypocalcemia might have an adverse effect on the neurological outcome of patients suffering from isolated severe TBI. In this study, we aimed to evaluate the relationship between admission Ca++ level and the neurological outcome of these patients.
Methods
This was a retrospective, single-center, cohort study of all patients admitted between January 2014 and December 2020 due to isolated severe TBI, which was defined as head abbreviated injury score ≥ 4 and an absence of severe (abbreviated injury score > 2) extracranial injuries. The primary outcome was a favorable neurological status at discharge, defined by a modified Rankin Scale of 0–2. Multivariable logistic regression was performed to determine whether admission hypocalcemia (Ca++ < 1.16 mmol L−1) is an independent predictor of neurological status at discharge.
Results
The final analysis included 201 patients. Hypocalcemia was common among patients with isolated severe TBI (73.1%). Most of the patients had mild hypocalcemia (1 < Ca++ < 1.16 mmol L−1), and only 13 (6.5%) patients had Ca++ ≤ 1.00 mmol L−1. In the entire cohort, hypocalcemia was independently associated with higher rates of good neurological status at discharge (adjusted odds ratio of 3.03, 95% confidence interval 1.11–8.33, p = 0.03). In the subgroup of 81 patients with an admission Glasgow Coma Scale > 8, 52 (64.2%) had hypocalcemia. Good neurological status at discharge was recorded in 28 (53.8%) of hypocalcemic patients compared with 14 (17.2%) of those with normal Ca++ (p = 0.002). In multivariate analyses, hypocalcemia was independently associated with good neurological status at discharge (adjusted odds ratio of 6.67, 95% confidence interval 1.39–33.33, p = 0.02).
Conclusions
Our study demonstrates that among patients with isolated severe TBI, mild admission hypocalcemia is associated with better neurological status at hospital discharge. The prognostic value of Ca++ may be greater among patients with admission Glasgow Coma Scale > 8. Trials are needed to investigate the role of hypocalcemia in brain injury.
Similar content being viewed by others
References
Gunning AC, Lansink KWW, Van Wessem KJP, et al. Demographic patterns and outcomes of patients in level i trauma centers in three international trauma systems. World J Surg. 2015;39(11):2677–84.
Scholten AC, Haagsma JA, Panneman MJM, Van Beeck EF, Polinder S. Traumatic brain injury in the Netherlands: incidence, costs and disability-adjusted life years. PLoS ONE. 2014;9(10): e110905.
Ma VY, Chan L, Carruthers KJ. The incidence, prevalence, costs and impact on disability of common conditions requiring rehabilitation in the US: stroke, spinal cord injury, traumatic brain injury, multiple sclerosis, osteoarthritis, rheumatoid arthritis, limb loss, and back pain. Arch Phys Med Rehabil. 2014;95(5):986.
Werner C, Engelhard K. Pathophysiology of traumatic brain injury. Br J Anaesth. 2007;99(1):4–9.
Wiles MD. Management of traumatic brain injury: a narrative review of current evidence. Anaesthesia. 2022;77(S1):102–12.
Zhang J, Zhang F, Dong JF. Coagulopathy induced by traumatic brain injury: systemic manifestation of a localized injury. Blood. 2018;131(18):2001.
Maas AIR, Menon DK, David Adelson PD, et al. Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research. Lancet Neurol. 2017;16(12):987–1048.
Helmrich IRAR, Czeiter E, Amrein K, et al. Incremental prognostic value of acute serum biomarkers for functional outcome after traumatic brain injury (CENTER-TBI): an observational cohort study. Lancet Neurol. 2022;21(9):792–802.
Magnotti LJ, Bradburn EH, Webb DL, et al. Admission ionized calcium levels predict the need for multiple transfusions: a prospective study of 591 critically ill trauma patient. J Trauma Acute Care Surg. 2011;70(2):391–7.
Ho KM, Yip CB. Concentration-dependent effect of hypocalcaemia on in vitro clot strength in patients at risk of bleeding: a retrospective cohort study. Transfus Med. 2016;26(1):57–62.
Vasudeva M, Mathew JK, Groombridge C, et al. Hypocalcemia in trauma patients: a systematic review. J Trauma Acute Care Surg. 2021;90(2):396–402.
Zhang P, Tu Q, Ni Z, et al. Association between serum calcium level and hemorrhagic progression in patients with traumatic intraparenchymal hemorrhage: investigating the mediation and interaction effects of coagulopathy. J Neurotrauma. 2022;39(7–8):508–19.
Weber JT. Altered calcium signaling following traumatic brain injury. Front Pharmacol 2012;3(60).
Gurkoff G, Shahlaie K, Lyeth B, Berman R. Voltage-gated calcium channel antagonists and traumatic brain injury. Pharm. 2013;6(7):788–812.
Weber J. Calcium homeostasis following traumatic neuronal injury. Curr Neurovasc Res. 2005;1(2):151–71.
Deshpande LS, Sun DA, Sombati S, et al. Alterations in neuronal calcium levels are associated with cognitive deficits after traumatic brain injury. Neurosci Lett. 2008;441(1):115–9.
Carney N, Totten AM, O’Reilly C, et al. Guidelines for the management of severe traumatic brain injury, fourth edition. Neurosurgery. 2017;80(1):6–15.
Guidelines for the management of severe traumatic brain injury. J Neurotrauma 2007;24 Suppl 1(supplement 1):i–vi.
Picetti E, Rossi S, Abu-Zidan FM, et al. WSES consensus conference guidelines: monitoring and management of severe adult traumatic brain injury patients with polytrauma in the first 24 hours. World J Emerg Surg. 2019;14(1):1–9.
Tiruneh A, Siman-Tov M, Givon A, et al. Comparison between traumatic brain injury with and without concomitant injuries: an analysis based on a national trauma registry 2008–2016. Brain Inj. 2020;34(2):213–23.
Kaji AH, Schriger D, Green S. Looking through the retrospectoscope: reducing bias in emergency medicine chart review studies. Ann Emerg Med. 2014;64(3):292–8.
von Elm E, Altman DG, Egger M, Pocock SJ, Gøtzsche PC, Vandenbroucke JP. The strengthening the reporting of observational studies in epidemiology (STROBE) statement: guidelines for reporting observational studies. Lancet. 2007;370(9596):1453–7.
Dubose JJ, Barmparas G, Inaba K, et al. Isolated severe traumatic brain injuries sustained during combat operations: demographics, mortality outcomes, and lessons to be learned from contrasts to civilian counterparts. J Trauma Acute Care Surg. 2011;70(1):11–8.
Talving P, Plurad D, Barmparas G, et al. Isolated severe traumatic brain injuries: association of blood alcohol levels with the severity of injuries and outcomes. J Trauma Acute Care Surg. 2010;68(2):357–62.
Hecht JP, LaDuke ZJ, Cain-Nielsen AH, Hemmila MR, Wahl WL. Effect of preinjury oral anticoagulants on outcomes following traumatic brain injury from falls in older adults. Pharmacother J Hum Pharmacol Drug Ther. 2020;40(7):604–13.
Quinn TJ, Dawson J, Walters MR, Lees KR. Functional outcome measures in contemporary stroke trials. Int J stroke Off J Int Stroke Soc. 2009;4(3):200–5.
Ganesh A, Luengo-Fernandez R, Wharton RM, Rothwell PM. Ordinal vs dichotomous analyses of modified rankin scale, 5-year outcome, and cost of stroke. Neurology. 2018;91(21):E1951–60.
P TM, Mat R. Assessing the utility of the modified Rankin scale (mRS) at discharge to predict day 90 outcomes in acute stroke registries. Circ Cardiovasc Qual Outcomes 2012;5(suppl_1):A168–A168.
Zelnick LR, Morrison LJ, Devlin SM, et al. Addressing the challenges of obtaining functional outcomes in traumatic brain injury research: missing data patterns, timing of follow-up, and three prognostic models. J Neurotrauma. 2014;31(11):1029.
Teasdale G, Jennett B. Assessment of coma and impaired consciousness. A practical scale. Lancet. 1974;304(7872):81–4.
Berry C, Ley EJ, Bukur M, et al. Redefining hypotension in traumatic brain injury. Injury. 2012;43(11):1833–7.
Davis PC. Head trauma. AJNR Am J Neuroradiol. 2007;28(8):1619.
Wandrup J, Kancir C, Petersen PH. Ionized calcium and acid-base status in arterial and venous whole blood during general anaesthesia. Scand J Clin Lab Invest. 1988;48(2):115–22.
Bilkovski RN, Cannon CM, Adhikari S, Nasr I. Arterial and venous ionized calcium measurements: Is there a difference? Ann Emerg Med. 2004;44(4):S56.
Perel PA, Olldashi F, Muzha I, et al. Predicting outcome after traumatic brain injury: practical prognostic models based on large cohort of international patients. BMJ. 2008;336(7641):425–9.
Steyerberg EW, Mushkudiani N, Perel P, et al. Predicting outcome after traumatic brain injury: development and international validation of prognostic scores based on admission characteristics. PLoS Med. 2008;5(8):1251–61.
Van Beek JGM, Mushkudiani NA, Steyerberg EW, et al. Prognostic value of admission laboratory parameters in traumatic brain injury: results from the IMPACT study. J Neurotrauma. 2007;24(2):315–28.
Moppett IK. Traumatic brain injury: assessment, resuscitation and early management. Br J Anaesth. 2007;99(1):18–31.
Roberts I, Shakur-Still H, Aeron-Thomas A, et al. Effects of tranexamic acid on death, disability, vascular occlusive events and other morbidities in patients with acute traumatic brain injury (CRASH-3): a randomised, placebo-controlled trial. Lancet. 2019;394(10210):1713–23.
Vasudeva M, Mathew JK, Fitzgerald MC, Cheung Z, Mitra B. Hypocalcaemia and traumatic coagulopathy: an observational analysis. Vox Sang. 2020;115(2):189–95.
Wray JP, Bridwell RE, Schauer SG, et al. The diamond of death: hypocalcemia in trauma and resuscitation. Am J Emerg Med. 2021;41:104–9.
Fineman I, Hovda DA, Smith M, Yoshino A, Becker DP. Concussive brain injury is associated with a prolonged accumulation of calcium: a 45Ca autoradiographic study. Brain Res. 1993;624(1–2):94–102.
Sun DA, Deshpande LS, Sombati S, et al. Traumatic brain injury causes a long-lasting calcium (Ca2+)-plateau of elevated intracellular Ca levels and altered Ca2+ homeostatic mechanisms in hippocampal neurons surviving brain injury. Eur J Neurosci. 2008;27(7):1672.
Mcintosh TK, Saatman KE, Raghupathi R. REVIEW: calcium and the pathogenesis of traumatic CNS injury: cellular and molecular mechanisms. Neurosci. 2016;3(3):169–75.
Vallentin MF, Granfeldt A, Meilandt C, et al. Effect of intravenous or intraosseous calcium vs saline on return of spontaneous circulation in adults with out-of-hospital cardiac arrest: a randomized clinical trial. JAMA. 2021;326(22):2268–76.
Wongtanasarasin W, Ungrungseesopon N, Namsongwong N, et al. Association between calcium administration and outcomes during adult cardiopulmonary resuscitation at the emergency department. Turkish J Emerg Med. 2022;22(2):67.
Choi DW. Glutamate neurotoxicity in cortical cell culture is calcium dependent. Neurosci Lett. 1985;58(3):293–7.
Han RZ, Hu JJ, Weng YC, Li DF, Huang Y. NMDA receptor antagonist MK-801 reduces neuronal damage and preserves learning and memory in a rat model of traumatic brain injury. Neurosci Bull. 2009;25(6):367–75.
Sönmez A, Sayin O, Gürgen SG, Çalişir M. Neuroprotective effects of MK-801 against traumatic brain injury in immature rats. Neurosci Lett. 2015;597:137–42.
Schlaepfer WW, Bunge RP. Effects of calcium ion concentration on the degeneration of amputated axons in tissue culture. J Cell Biol. 1973;59(2 Pt 1):456–70.
Bailey I, Bell A, Gray J, et al. A trial of the effect of nimodipine on outcome after head injury. Acta Neurochir (Wien). 1991;110(3–4):97–105.
Murray GD, Teasdale GM, Schmitz H. Nimodipine in traumatic subarachnoid haemorrhage: a re-analysis of the HIT I and HIT II trials. Acta Neurochir (Wien). 1996;138(10):1163–7.
Langham J, Goldfrad C, Teasdale G, Shaw D, Rowan K. Calcium channel blockers for acute traumatic brain injury. Cochrane Database Syst Rev 2003;(4).
DI Vergouwen M, Vermeulen M, Roos YB. Effect of nimodipine on outcome in patients with traumatic subarachnoid haemorrhage: a systematic review. Lancet Neurol. 2006;5(12):1029–32.
Morotti A, Charidimou A, Phuah CL, et al. Association between serum calcium level and extent of bleeding in patients with intracerebral hemorrhage. JAMA Neurol. 2016;73(11):1285–90.
Tu L, Liu X, Li T, et al. Admission serum calcium level as a prognostic marker for intracerebral hemorrhage. Neurocrit Care. 2019;30(1):81–7.
Zhang YB, Zheng SF, Yao PS, et al. Lower ionized calcium predicts hematoma expansion and poor outcome in patients with hypertensive intracerebral hemorrhage. World Neurosurg. 2018;118:e500-4.
Epstein D, Freund Y, Marcusohn E, et al. Association between ionized calcium level and neurological outcome in endovascularly treated patients with spontaneous subarachnoid hemorrhage: a retrospective cohort study. Neurocrit Care 2021; 1–15.
Adatia K, Newcombe VFJ, Menon DK. Contusion progression following traumatic brain injury: a review of clinical and radiological predictors, and influence on outcome. Neurocrit Care. 2021;34(1):312.
Martin S-A, Juan S-R, Fernando M-A, et al. Hypocalcemia as a prognostic factor in mortality and morbidity in moderate and severe traumatic brain injury. Asian J Neurosurg. 2015;10(3):190.
Vinas-Rios JM, Sanchez-Aguilar M, Sanchez-Rodriguez JJ, et al. Hypocalcaemia as a prognostic factor of early mortality in moderate and severe traumatic brain injury. Neurol Res. 2014;36(2):102–6.
van Gent JAN, van Essen TA, Bos MHA, Cannegieter SC, van Dijck JTJM, Peul WC. Coagulopathy after hemorrhagic traumatic brain injury, an observational study of the incidence and prognosis. Acta Neurochir (Wien). 2020;162(2):329–36.
Foreman BP, Caesar RR, Parks J, et al. Usefulness of the abbreviated injury score and the injury severity score in comparison to the Glasgow Coma Scale in predicting outcome after traumatic brain injury. J Trauma Acute Care Surg. 2007;62(4):946–50.
Funding
None.
Author information
Authors and Affiliations
Contributions
KB has contributed to the design of the research, the acquisition of data and drafting the article. NH has contributed to acquisition of data, EA has contributed to the design of the research and the acquisition of data, HB has contributed to the conception of research and acquisition of data. YB and AR has revised the data. MR has contributed to the design of the research, analysis and interpretation of data and drafting the article. DE has contributed to the conception, analysis, and interpretation of data and drafting the article. The final manuscript was approved by all authors.
Corresponding author
Ethics declarations
Conflict of interest
Aeyal Raz reports receiving consultant fees Medtronic and Neuroindex and speaker’s fee from MSD (none related to this work). All the authors declare that they have no conflicts of interest.
Ethical approval/informed consent
The study was performed in concordance with the Helsinki declaration and was approved by the Institutional Review Board at Rambam Health Care Campus (Approval Number 0184-22-RMB-D). The need for written informed consent was waived because of the retrospective study design.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Badarni, K., Harush, N., Andrawus, E. et al. Association Between Admission Ionized Calcium Level and Neurological Outcome of Patients with Isolated Severe Traumatic Brain Injury: A Retrospective Cohort Study. Neurocrit Care 39, 386–398 (2023). https://doi.org/10.1007/s12028-023-01687-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12028-023-01687-4