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The Role of MiR-375 in Migration and Invasion of H.pylori-induced Gastric Cancer Cell Model

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Abstract

This article aimed to investigate the mechanism of miR-375 in Hp-induced gastric cancer cells (GCCs) model. Human normal gastric mucosal epithelial cell (GMEC) line GES-1 and human GCCs strain MKN45 were used as research objects. The expression of miR-375 was detected after H.pylori (Hp) infection of GCCs. The cell activity was detected by, 53-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the cell multiplication was determined by cell counting kit-8 (CCK-8) method. Transwell assay was used to detect the effect of cell invasion and migration ability. The expression levels of JAK1 and STAT3 proteins were determined by Western blot (WB). MiR-375 was increased in GCCs after Hp infection, and JAK1, STAT3, p-JAK1, and p-STAT3 in GCCs after Hp infection were visibly increased. In addition, the overexpressed miR-375 promoted the multiplication activity, migration, and invasion ability of GCCs. MiR-375 promotes Hp-induced migration and invasion of GCCs by targeting JAK1/STAT3. This article reveals the important role of miR-375 in Hp-induced GC, which provides new clues for further study of its mechanism and therapeutic targets.

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Authors and Affiliations

  1. Department of Gastroenterology, Yiling People’s Hospital, Yichang, Hubei Province, China

    Zhichao Mao, Xinyu Wang, Yongtang Zhao, Fei Yang & Qin Qin

  2. Department of Cardiovascular, Yiling People’s Hospital, Yichang, Hubei Province, China

    Ruilian Jiang

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Z.M., X.W., Y.Z., F.Y., Q.Q., and R.J. participated the search and collection data, drafting of the manuscript; study concept and design, study supervision. All authors read and approved the final manuscript.

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Correspondence to Ruilian Jiang.

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Mao, Z., Wang, X., Zhao, Y. et al. The Role of MiR-375 in Migration and Invasion of H.pylori-induced Gastric Cancer Cell Model. Cell Biochem Biophys 83, 429–435 (2025). https://doi.org/10.1007/s12013-024-01473-9

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