Abstract
Tri-Circulator (TC) is a product comprising coenzyme Q10 (CoQ10), Salvia miltiorrhiza, and Panax notoginseng. Individually, each of these constituents has demonstrated protective effects on myocardial injury. The purpose of this study is to evaluate the protective efficacy of TC on heart function and compare the differential effects between CoQ10 and TC. Two myocardial injury models of zebrafish, the hypoxia-reoxygenation model (H/R) and the isoproterenol (ISO, a β-receptor agonist) model, were used in this experiment. The zebrafish subjects were divided into 4 groups: control, H/R, TC, and CoQ10. Heart rate, stroke volume (SV), cardiac output (CO), ejection fraction (EF), fractional area change (FAC), and pericardial height were monitored to assess changes in heart function. The gene expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) was studied as markers of injury/stress. TC significantly suppresses elevated heart rate induced by H/R and prevents the decrease of heart rate induced by ISO. It alleviates the pericardial infusion induced by ISO, whereas CoQ10 does not possess a similar effect. Both TC and CoQ10 significantly inhibit the decline in SV, CO, EF, and FAC induced by H/R and ISO, and suppress the expression of ANP and BNP in cardiomyocytes induced by ISO. It is noteworthy that TC demonstrates a more pronounced effect on EF, FAC, ANP, and BNP gene expression compared to CoQ10. Both TC and CoQ10 have a protective effect on myocardial injury of zebrafish. However, TC exhibits a greater efficacy compared to CoQ10 alone in mitigating myocardial injury.
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Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.
Abbreviations
- ANP:
-
Atrial natriuretic peptide
- BNP:
-
Brain natriuretic peptide
- CoQ10:
-
Coenzyme Q10
- CO:
-
Cardiac output
- DPF:
-
Day post fertilization
- EF:
-
Ejection fraction
- FAC:
-
Fractional area change
- H/R:
-
Hypoxia-reoxygenation
- ISO:
-
Isoproterenol
- PN:
-
Panax notoginseng
- SM:
-
Salvia miltiorrhiza
- SV:
-
Stroke-volume
- TC:
-
Tri-circulator
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Acknowledgements
This work is supported by TCM Health Education & Research Center, USA.
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YX, RW, and SK contributed toward conceptualization, writing–review & editing, formal analysis, writing–original draft, and methodology. TZ contributed toward formal analysis, and writing–original draft. YX contributed toward methodology and software. YS contributed toward validation. HN contributed toward conceptualization, writing–review & editing, funding acquisition, supervision, methodology, and project administration.
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Xiao, Y., Wang, R., Kong, S. et al. Comparison of Protective Effect of Tri-circulator and Coenzyme Q10 on Myocardial Injury and the Mechanism Study by Zebrafish Model. Cardiovasc Toxicol 24, 258–265 (2024). https://doi.org/10.1007/s12012-024-09828-7
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DOI: https://doi.org/10.1007/s12012-024-09828-7