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Relationship Between a High-Fat Diet, Reduced Mobility, and Trace Element Overload in the Olfactory Bulbs of C57BL/6J and DBA/2J Mice

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Abstract

The dysregulation of trace elements in the brain, which can be caused by genetic or environmental factors, has been associated with disease and compromised mobility. Research regarding trace elements and motor function has focused mainly on the basal ganglia, but few studies have examined the olfactory bulb in this context. Diets high in fat have been shown to have consequences of dysregulated iron and manganese in the brain and disrupted motor activity. The aim of our study was to examine the relationship between mobility and trace element disruption in the olfactory bulb in male and female C57BL/6J and DBA/2J mice fed a high-fat diet. Mobility was significantly reduced in male C57BL/6Js, but the correlation between iron and manganese in the olfactory bulb with velocity, distance travelled, and habituation was not statistically significant. However, there appears to be an overall pattern of a high-fat diet having a statistically significant impact individually on elevated iron and manganese in the olfactory bulb, reduced velocity, reduced distance travelled, and reduced habituation mainly in the male C57BL/6J strain. We found similar trends within the scientific literature to suggest that dysregulated trace element status in the olfactory bulb may be related to motor function in both humans and animals and that males may be more susceptible to the negative outcomes. Our findings contribute new information regarding the impact of diet on the brain, behavior, and potential connection between trace element dysregulation in the olfactory bulb with mobility.

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Supporting data for this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors acknowledge Paula Cooney for assistance with tissue collection and Mary Martinez for assistance with animal care.

Funding

This research was funded by the UNC Greensboro Health and Human Sciences Research Grant and Faculty First Award.

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Authors and Affiliations

  1. Department of Chemistry and Physics, Salem College, Winston-Salem, NC, USA

    Melissa S. Totten & Jenna M. Howell

  2. Department of Biology, Salem College, Winston-Salem, NC, USA

    Jordan A. Tomberlin

  3. Department of Nutrition, University of North Carolina at Greensboro, Greensboro, NC, USA

    Keith M. Erikson

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  1. Melissa S. Totten
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Correspondence to Melissa S. Totten.

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This study was conducted in an American Association for Laboratory Animal Care accredited facility following a protocol approved by the Institution of Animal Care and Use Committee at the University of North Carolina Greensboro (protocol #18 − 001). Procedures were performed by the principles and guidelines established by the National Institutes of Health for the ethical care and use of laboratory animals.

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Totten, M.S., Howell, J.M., Tomberlin, J.A. et al. Relationship Between a High-Fat Diet, Reduced Mobility, and Trace Element Overload in the Olfactory Bulbs of C57BL/6J and DBA/2J Mice. Biol Trace Elem Res 202, 3215–3224 (2024). https://doi.org/10.1007/s12011-023-03911-w

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