Abstract
Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O3, and As + O3. As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) O3 in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O3 and O3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue.
In TUNEL assessments, similar outcomes were obtained in the control and As + O3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O3 and As + O3 groups. There was no significant difference between the As + O3 and O3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O3 group compared to the As + O3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.
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The data supporting this study’s findings are available from the corresponding author upon request.
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All authors contributed to the study’s conception and design. Material preparation and data collection were performed by Hüseyin Avni Eroğlu, Cemre Aydeğer, and Mustafa Makav. Histopathological and TUNEL analyses were performed by Başak Büyük. Genetic analyses were performed by Özlem Öztopuz and Mehmet Akif Ovalı. The first draft of the manuscript was written by Hüseyin Avni Eroğlu and Başak Büyük. All authors read and approved the final manuscript.
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Büyük, B., Aydeğer, C., Öztopuz, Ö. et al. Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats. Biol Trace Elem Res 201, 3951–3960 (2023). https://doi.org/10.1007/s12011-022-03486-y
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DOI: https://doi.org/10.1007/s12011-022-03486-y