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Evaluation of Copper Levels in Dental Calculus of OSF Patients with Chewing Dried Areca-Nut Quids in Hunan Province of Mainland China

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Abstract

Dental calculus is a potential material that can be used for assessing chronic exposure to trace heavy metals in oral cavity as it is a long-term reservoir. The aim of this study was to investigate the correlation between dental calculus copper levels and risk of oral submucous fibrosis (OSF) due to chewing dried areca-nut quids in Mainland China. This study included 34 OSF (grade 1) sufferers with dried areca-nut quids chewing as the patient group and 23 healthy individuals without areca-nut chewing as the control group. The dental calculus sample was obtained from all 57 participants and evaluated by inductively coupled plasma mass spectrometry (ICP-MS) for dental calculus level of copper. This work revealed that the mean copper level of dental calculus was significantly higher in OSF (grade 1) sufferers with areca-nut chewing than those in healthy individuals without areca-nut chewing (p < 0.001). This work provided an evidence to support that there may be a positive correlation between elevated levels of copper in dental calculus caused by chewing dried areca-nut quids and an increased risk of developing OSF in Mainland China.

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Data Availability

The data that support the findings of this study are not openly available and are available from the corresponding author upon reasonable request.

References

  1. Jian X, Jian Y, Wu X, Guo F, Hu Y, Gao X, Jiang C, Li N, Wu Y, Liu D (2021) Oral submucous fibrosis transforming into squamous cell carcinoma: a prospective study over 31 years in mainland China. Clin Oral Investig 25(4):2249–2256. https://doi.org/10.1007/s00784-020-03541-9

    Article  Google Scholar 

  2. Arakeri G, Rai KK, Hunasgi S, Merkx MAW, Gao S, Brennan PA (2017) Oral submucous fibrosis: an update on current theories of pathogenesis. J Oral Pathol Med 46(6):406–412. https://doi.org/10.1111/jop.12581

    Article  Google Scholar 

  3. Cheng RH, Wang YP, Chang JY, Pan YH, Chang MC, Jeng JH (2020) Genetic susceptibility and protein expression of extracellular matrix turnover-related genes in oral submucous fibrosis. Int J Mol Sci 21(21):8104. https://doi.org/10.3390/ijms21218104

    Article  CAS  Google Scholar 

  4. Jani YV, Chaudhary AR, Dudhia BB, Bhatia PV, Soni NC, Patel PS (2017) Evaluation of role of trace elements in oral submucous fibrosis patients: a study on Gujarati population. J Oral Maxillofac Pathol 21(3):455. https://doi.org/10.4103/jomfp.JOMFP_106_14

    Article  Google Scholar 

  5. Radhika T, Sekaran P, Narasimhan M (2016) Qualitative analysis of collagen fibers in oral submucous fibrosis using picrosirius red stain and polarising microscope. J Clin Diagn Res 10(2):ZC04–7. https://doi.org/10.7860/JCDR/2016/13729.7164

  6. Thakkannavar SS, Naik VV (2019) Histochemical and immunohistochemical analysis of collagen fibers and microvascular density in various grades of oral submucous fibrosis. Iran J Pathol 14(2):127–134. https://doi.org/10.30699/IJP.14.2.127

    Article  Google Scholar 

  7. Tilakaratne WM, Ekanayaka RP, Warnakulasuriya S (2016) Oral submucous fibrosis: a historical perspective and a review on etiology and pathogenesis. Oral Surg Oral Med Oral Pathol Oral Radiol 122(2):178–191. https://doi.org/10.1016/j.oooo.2016.04.003

    Article  Google Scholar 

  8. Trivedy CR, Warnakulasuriya KA, Peters TJ, Senkus R, Hazarey VK, Johnson NW (2000) Raised tissue copper levels in oral submucous fibrosis. J Oral Pathol Med 29(6):241–248. https://doi.org/10.1034/j.1600-0714.2000.290601.x

    Article  CAS  Google Scholar 

  9. Pillai KG, Burde KN (2005) Increased copper level in oral mucosal tissue of patients with submucous fibrosis and who chew areca nut products. West Indian Med J 54(4):270–271. https://doi.org/10.1590/s0043-31442005000400014

    Article  CAS  Google Scholar 

  10. Akcali A (2000) Lang NP (2018) Dental calculus: the calcified biofilm and its role in disease development. Periodontol 76(1):109–115. https://doi.org/10.1111/prd.12151

    Article  Google Scholar 

  11. Yaprak E, Yolcubal I, Sinanoğlu A et al (2017) High levels of heavy metal accumulation in dental calculus of smokers: a pilot Inductively coupled plasma mass spectrometry study. J Periodont Res 52(1):83–88. https://doi.org/10.1111/jre.12371

    Article  CAS  Google Scholar 

  12. Zhang B, Tan XD, Zhang KL (2018) Cadmium profiles in dental calculus: a cross-sectional population-based study in Hunan province of China. Biol Trace Elem Res 185(1):63–70. https://doi.org/10.1007/s12011-018-1251-z

    Article  CAS  Google Scholar 

  13. Zhang B, Tan XD, He XF et al (2019) Evaluation of cadmium levels in dental calculus of male oral SCC patients with betel-quid chewing in Hunan province of China. Biol Trace Elem Res 191(2):348–353. https://doi.org/10.1007/s12011-019-1639-4

    Article  CAS  Google Scholar 

  14. Sørensen LK, Hasselstrøm JB, Larsen LS, Bindslev DA (2021) Entrapment of drugs in dental calculus-detection validation based on test results from post-mortem investigations. Forensic Sci Int 319:110647. https://doi.org/10.1016/j.forsciint.2020.110647

    Article  CAS  Google Scholar 

  15. Pranata N, Maskoen AM, Sahiratmadja E, Widyaputra S (2020) Dental calculus as a potential biosource for human papillomavirus detection in oral squamous cell carcinoma. Asian Pac J Cancer Prev 21(10):3093–3097. https://doi.org/10.31557/APJCP.2020.21.10.3093

    Article  CAS  Google Scholar 

  16. Metcalf JL, Ursell LK, Knight R (2014) Ancient human oral plaque preserves a wealth of biological data. Nat Genet 46(4):321–323. https://doi.org/10.1038/ng.2930

    Article  CAS  Google Scholar 

  17. Warinner C, Rodrigues JF, Vyas R, Trachsel C, Shved N et al (2014) Pathogens and host immunity in the ancient human oral cavity. Nat Genet 46(4):336–344. https://doi.org/10.1038/ng.2906

    Article  CAS  Google Scholar 

  18. Costa de Almeida GR, Pereira Saraiva Mda C, Barbosa F Jr, Krug FJ, Cury JA et al (2007) Lead contents in the surface enamel of deciduous teeth sampled in vivo from children in uncontaminated and in lead-contaminated areas. Environ Res 104(3):337–345. https://doi.org/10.1016/j.envres.2007.03.007

    Article  CAS  Google Scholar 

  19. Mathew P, Austin RD, Varghese SS, Manojkumar, (2014) Estimation and comparison of copper content in raw areca nuts and commercial areca nut products: implications in increasing prevalence of oral submucous fibrosis (OSMF). J Clin Diagn Res 8(1):247–249. https://doi.org/10.7860/JCDR/2014/8042.3932

    Article  Google Scholar 

  20. Trivedy C, Baldwin D, Warnakulasuriya S, Johnson N, Peters T (1997) Copper content in areca catechu (betel nut) products and oral submucous fibrosis. Lancet 349(9063):1447. https://doi.org/10.1016/S0140-6736(97)24020-1

    Article  CAS  Google Scholar 

  21. Patil S, Maheshwari S (2014) Proposed new grading of oral submucous fibrosis based on cheek flexibility. J Clin Exp Dent 6(3):e255-258. https://doi.org/10.4317/jced.51378

    Article  Google Scholar 

  22. Paul RR, Chatterjee J, Das AK, Cervera ML, de la Guardia M, Chaudhuri K (2002) Altered elemental profile as indicator of homeostatic imbalance in pathogenesis of oral submucous fibrosis. Biol Trace Elem Res 87(1–3):45–56. https://doi.org/10.1385/BTER:87:1-3:045

    Article  CAS  Google Scholar 

  23. Khan S, Chatra L, Prashanth SK, Veena KM, Rao PK (2012) Pathogenesis of oral submucous fibrosis. J Cancer Res Ther 8(2):199–203. https://doi.org/10.4103/0973-1482.98970

    Article  Google Scholar 

  24. Sharma A, Sahni P, Nayak MT, Singhvi A, Kumar R (2014) Identification of the pattern of copper as an etiological factor in oral submucous fibrosis: a cytological study. J Exp Ther Oncol 10(4):317–323

    Google Scholar 

  25. Alexander AJ, Ramani P, Sherlin HJ, Gheena S (2019) Quantitative analysis of copper levels in areca nut plantation area - a role in increasing prevalence of oral submucous fibrosis: an in vitro study. Indian J Dent Res 30(2):261–266. https://doi.org/10.4103/ijdr.IJDR_431_17

    Article  Google Scholar 

  26. Mathew P, Austin RD, Varghese SS, Manojkumar AD (2015) Effect of copper-based fungicide (bordeaux mixture) spray on the total copper content of areca nut: implications in increasing prevalence of oral submucous fibrosis. J Int Soc Prev Community Dent 5(4):283–289. https://doi.org/10.4103/2231-0762.161755

    Article  Google Scholar 

  27. Velsko IM, Overmyer KA, Speller C, Klaus L, Collins MJ, Loe L, Frantz LAF, Sankaranarayanan K, Lewis CM Jr, Martinez JBR, Chaves E, Coon JJ, Larson G, Warinner C (2017) The dental calculus metabolome in modern and historic samples. Metabolomics 13(11):134. https://doi.org/10.1007/s11306-017-1270-3

    Article  CAS  Google Scholar 

  28. Chen X, He Y, Deng Y (2021) Chemical composition, pharmacological, and toxicological effects of betel nut. Evid Based Complement Alternat Med 2021:1808081. https://doi.org/10.1155/2021/1808081

    Article  Google Scholar 

  29. Guengerich FP (2018) Introduction to metals in biology 2018: copper homeostasis and utilization in redox enzymes. J Biol Chem 293(13):4603–4605. https://doi.org/10.1074/jbc.TM118.002255

    Article  CAS  Google Scholar 

  30. Pajarillo EAB, Lee E, Kang DK (2021) Trace metals and animal health: Interplay of the gut microbiota with iron, manganese, zinc, and copper. Anim Nutr 7(3):750–761. https://doi.org/10.1016/j.aninu.2021.03.005

    Article  CAS  Google Scholar 

  31. Shen YW, Shih YH, Fuh LJ, Shieh TM (2020) Oral submucous fibrosis: a review on biomarkers, pathogenic mechanisms, and treatments. Int J Mol Sci 21(19):7231. https://doi.org/10.3390/ijms21197231

    Article  CAS  Google Scholar 

  32. Khan I, Pant I, Narra S, Radhesh R, Ranganathan K, Rao SG, Kondaiah P (2015) Epithelial atrophy in oral submucous fibrosis is mediated by copper (II) and arecoline of areca nut. J Cell Mol Med 19(10):2397–2412. https://doi.org/10.1111/jcmm.12622

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to thank Prof. Tian-Ting Zhang, at The Third Xiangya Hospital, Changsha, Hunan, PR China, for encouragement and support for this study.

Funding

This study was supported by Hunan S & T Project of China Hunan provincial science and technology department (Grant No. 2014FJ3032).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Hunan Provincial People’s Hospital and The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan, People’s Republic of China

    Xi-Fan He, Te Zhang, Xin-Lan Yan, Yi-Wei Wu, Yi-Sha Pan, Yang-Bo Ning & Michael Bo Zhang

  2. Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China

    Xi-Fan He & Lin Chen

  3. Department of Stomatology, First Affiliated Hospital of Hunan College of Traditional Chinese Medicine (Zhuzhou Hospital of Traditional Chinese Medicine), Zhuzhou, People’s Republic of China

    Xi-Fan He

  4. Changsha Institute for Food and Drug Control & National Alcohol Products Quality Supervision and Inspection Center, Changsha, Hunan, People’s Republic of China

    Hui Wang, Zhi-Peng Qiu, Xiao-Jiao Cui & Jing-Sha Zhou

  5. Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People’s Republic of China

    Ye Tian

  6. Hanwoo DNA Solutions Co., Ltd, Changsha, People’s Republic of China

    Kun-Lun Zhang, Wei-Hao Zhao, David Sharma, Xiao-Dan Tan & Michael Bo Zhang

  7. Hunan Legal Forensic Center, Changsha, People’s Republic of China

    Xiao-Dan Tan & Michael Bo Zhang

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  1. Xi-Fan He
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Contributions

Xi-Fan He, Hui Wang, Te Zhang, Ye Tian, and Xiao-Dan Tan contributed equally to this work. Michael Bo Zhang, Xiao-Dan Tan, and Xi-Fan He participated in the study design. Michael Bo Zhang, Xi-Fan He, Te Zhang, Xin-Lan Yan, Yi-Wei Wu, Yi-Sha Pan, and Yang-Bo Ning collected dental calculus samples. Michael Bo Zhang, Hui Wang, Zhi-Peng Qiu, Xiao-Jiao Cui, Xiao-Dan Tan, Kun-Lun Zhang, and Wei-Hao Zhao performed experiments. Michael Bo Zhang, Xi-Fan He, and Lin Chen analyzed data. Michael Bo Zhang, Xiao-Dan Tan, David Sarma, Hui Wang, Ye Tian, and Xi-Fan He wrote the paper. All authors read and approved the paper.

Corresponding author

Correspondence to Michael Bo Zhang.

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Ethical Approval

This study was approved by the Ethics Committee of Hunan Provincial People’s Hospital.

Informed Consent

Informed and signed consent was obtained from all individual participants enrolled in this study.

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The authors declare no competing interests.

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He, XF., Wang, H., Tian, Y. et al. Evaluation of Copper Levels in Dental Calculus of OSF Patients with Chewing Dried Areca-Nut Quids in Hunan Province of Mainland China. Biol Trace Elem Res 201, 677–682 (2023). https://doi.org/10.1007/s12011-022-03210-w

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