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The Effect of Zinc Sulfate on miR-122, miR-34a, Atioxidants, Biochemical and Histopathological Parameters Following Hepatic Ischemia/Reperfusion Injury in Rats

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Abstract

Liver ischemia-reperfusion (IR) injury is a situation which occurs in various conditions such as pringle maneuver and liver transplantation. The regulatory effect of zinc sulfate (ZnSO4) is an important trace element on several liver disorders well known, but its effects on microRNAS (miR-122 and miR-34a) have not been evaluated. The goal of this study was to identify the protective effects of ZnSO4 on IR-induced liver injury, in particular, microRNAS in rats. Thirty-two male Wistar rats were randomly assigned into four groups (eight each group): sham, IR, ZnSO4 pretreatment, and ZnSO4 + IR groups. In sham and ZnSO4 pretreatment groups, animals received normal saline (N/S, 2 ml/kg) and ZnSO4 (5 mg/kg) for 7 consecutive days intraperitoneally (ip), then only laparotomy was performed. In IR and ZnSO4 + IR groups, N/S and ZnSO4, respectively, were given with the same dose, time, and route, before induction of ischemia for 45 min followed by reperfusion for 60 min. Blood sample was taken for biochemical and microRNAs analysis. Tissue specimens also were obtained for the measurements of antioxidant activities and histopathological evaluations. Our results showed that ZnSO4 pretreatment ameliorated histopathological changes decreased the increased serum levels of liver enzymes, miR-122 and miR-34a, and enhanced the decreased activity of antioxidant enzymes following hepatic IR injury. The present study indicated that ZnSO4 had potential hepatoprotective action against IR-induced injury. Therefore, it has been suggested that it can be administered as an anti-miR before elective hepatic surgeries for prevention of this complication.

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Acknowledgments

The source of data used in this paper was from the Ph.D thesis of Mrs Ghaidafeh Akbari, a student of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. The authors gratefully acknowledge the help of the Physiology Research Center of Ahvaz Jundishapur University of Medical Sciences.

Funding

This study received financial support from the Physiology Research Center of Ahvaz Jundishapur University of Medical Sciences (No: APRC-9421).

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Authors and Affiliations

  1. Physiology Research Center (PRC), Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Seyyed Ali Mard & Ghaidafeh Akbari

  2. Yasuj University of Medical Sciences, Medical sciences, Yasuj, Iran

    Ghaidafeh Akbari

  3. Physiology Research Center (PRC), Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Mahin Dianat

  4. Cellular and Molecular Research Center, Department of Anatomic Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Esrafil Mansouri

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  1. Seyyed Ali Mard
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Correspondence to Ghaidafeh Akbari.

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All procedures were done in accordance with the ethics committee of Ahvaz Jundishapur University of Medical Sciences.

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Mard, S.A., Akbari, G., Dianat, M. et al. The Effect of Zinc Sulfate on miR-122, miR-34a, Atioxidants, Biochemical and Histopathological Parameters Following Hepatic Ischemia/Reperfusion Injury in Rats. Biol Trace Elem Res 188, 434–440 (2019). https://doi.org/10.1007/s12011-018-1425-8

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