Abstract
The aim of the present study was to determine changes occurring in serum and urine concentrations of essential trace elements with proven essentiality (molybdenum, selenium, and zinc) as a result of performing an acute physical activity until exhaustion in middle- and long-distance runners who live in the same area of Extremadura (Spain). Twenty-one Spanish national middle- and long-distance runners and 26 sedentary students of a similar age were recruited for the study. Both groups ran on a treadmill until exhaustion, starting at a speed of 10 and 8 km/h, respectively, and increasing the speed at 1 km/h every 400 m, without modifying the slope, always within the recommended parameters. Serum and urine samples were obtained from all subjects before and after the tests. Analysis of trace metals was performed by inductively coupled plasma mass spectrometry (ICP-MS). Resting serum and urinary concentrations between groups were compared using the Student t test, and the Wilcoxon test was used to analyze the trends of changes before and after the effort. The results showed that molybdenum concentrations were significantly higher in athletes than in controls (p < 0.01). Selenium (p < 0.05) and zinc (p < 0.01) concentrations were significantly lower in athletes than in controls. When we compared the serum concentrations before and after the test in the controls, only in the case of selenium (p = 0.006), a significant increase was observed after the test. However, this signification disappears with the corrections for hematocrit. Athletes’ serum concentrations of Se (p = 0.004) and Zn (p = 0.005) lowered at the end of the test. Also, the results showed that there were no statistical urinary concentration (expressed in μg/g creatinine) changes in Mo and Se. Zn urinary concentration increased at the end of exercise (p = 0.018), since an incremental exercise to exhaustion in middle- and long-distance elite athletes produces a decrease in Se and Zn serum concentrations but Zn urinary concentration increased. In conclusion, athletes show higher serum concentrations of molybdenum and lower serum concentrations of selenium and zinc than sedentary subjects. Additionally, a treadmill test until exhaustion provokes a decrease in serum concentration of selenium and zinc and a higher excretion of urinary zinc. Serum concentrations of Se and Zn should be carried out in order to avoid any possible deficit cases and to establish the optimal supplementation.
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LLerena F, Maynar M, Barrientos G, Palomo R, Robles MC, Caballero MJ (2012) Comparison of urine toxic metals concentrations in athletes and in sedentary subjects living in the same area of Extremadura (Spain). Eur J Appl Physiol 112:3027–3031. https://doi.org/10.1007/s00421-011-2276-6
Maynar M, Llerena F, Grijota FJ, Alves J, Robles MC, Bartolomé I, Muñoz D (2017) Serum concentration of several trace metals and physical training. J Int Soc Sports Nutr 14:19. https://doi.org/10.1186/s12970-017-0178-7
Rodríguez Tuya I, Pinilla Gil E, Maynar Mariño M et al (1996) Evaluation of the influence of physical activity on the plasma concentrations of several trace metals. Eur J Appl Physiol Occup Physiol 73:299–303
Döker S, Hazar M, Uslu M, Okan İ, Kafkas E, Boşgelmez İİ (2014) Influence of training frequency on serum concentrations of some essential trace elements and electrolytes in male swimmers. Biol Trace Elem Res 158:15–21. https://doi.org/10.1007/s12011-014-9912-z
Prashanth L, Kattapagari K, Chitturi R et al (2015) A review on role of essential trace elements in health and disease. J Dr NTR Univ Heal Sci 4:75. https://doi.org/10.4103/2277-8632.158577
Akil M, Gurbuz U, Bicer M, Sivrikaya A, Mogulkoc R, Baltaci AK (2011) Effect of selenium supplementation on lipid peroxidation, antioxidant enzymes, and lactate levels in rats immediately after acute swimming exercise. Biol Trace Elem Res 142:651–659. https://doi.org/10.1007/s12011-010-8785-z
Bicer M, Gunay M, Baltaci AK, Uney K, Mogulkoc R, Akil M (2012) Effect of zinc supplementation on lipid peroxidation and lactate levels in rats with diabetes induced by streptozotocin and subjected to acute swimming exercise. Bratisl Lek Listy 113:199–205
Hazar M (2009) Effectd of intense endurance exercise on serum levels of zinc and copper in elite rowers. Asian J Chem 21:567
Marrella M, Guerrini F, Solero PL, Tregnaghi PL, Schena F, Velo GP (1993) Blood copper and zinc changes in runners after a marathon. J Trace Elem Electrolytes Health Dis 7:248–250
Flinn JM, Hunter D, Linkous DH et al (2005) Enhanced zinc consumption causes memory deficits and increased brain levels of zinc. Physiol Behav 83:793–803. https://doi.org/10.1016/J.PHYSBEH.2004.10.009
Cordova A, Alvarez-Mon M (1995) Behaviour of zinc in physical exercise: a special reference to immunity and fatigue. Neurosci Biobehav Rev 19:439–445
Van Loan MD, Sutherland B, Lowe NM et al (1999) The effects of zinc depletion on peak force and total work of knee and shoulder extensor and flexor muscles. Int J Sport Nutr 9:125–135
Baltaci AK, Mogulkoc R, Akil M, Bicer M (2016) Review—selenium—its metabolism and relation to exercise. Pak J Pharm Sci 29:1719–1725
Gojanovic B, Cutti P, Shultz R, Matheson G (2013) High intensity interval training at overspeed in a lower body positive pressure treadmill improves performance in trained runners. J Sci Med Sport 16:e29. https://doi.org/10.1016/j.jsams.2013.10.070
Kabata-Pendias A, Mukherjee A (2007) Trace elements from soil to human. Springer, Heidelberg
Reilly C (2004) The nutritional trace metals. Blackwell Publishing Ltd, Oxford
Moreiras O (2016) Tablas de composición de alimentos : guía de prácticas. Pirámide, Madrid
Niemelä K, Palatsi I, Takkunen J (1980) The oxygen uptake—work-output relationship of runners during graded cycling exercise: sprinters vs. endurance runners. Br J Sports Med 14:204–209. https://doi.org/10.1136/BJSM.14.4.204
Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248. https://doi.org/10.1152/jappl.1974.37.2.247
Shi H, Ma Y, Ma Y (1995) A simple and fast method to determine and quantify urinary creatinine. Anal Chim Acta 312:79–83. https://doi.org/10.1016/0003-2670(95)00208-H
Heitland P, Köster HD (2004) Fast, simple and reliable routine determination of 23 elements in urine by ICP-MS. J Anal At Spectrom 19:1552–1558. https://doi.org/10.1039/B410630J
Heitland P, Köster HD (2006) Biomonitoring of 30 trace elements in urine of children and adults by ICP-MS. Clin Chim Acta 365:310–318. https://doi.org/10.1016/J.CCA.2005.09.013
Lu Y, Ahmed S, Harari F, Vahter M (2015) Impact of Ficoll density gradient centrifugation on major and trace element concentrations in erythrocytes and blood plasma. J Trace Elem Med Biol 29:249–254. https://doi.org/10.1016/J.JTEMB.2014.08.012
Berger CE, Kröner A, Kluger R, Baron R, Steffan I, Engel A (2002) Effects of marathon running on the trace minerals chromium, cobalt, nickel, and molybdenum. J Trace Elem Exp Med 15:201–209. https://doi.org/10.1002/jtra.10019
Rodas G, Ventura JL, Cadefau JA, Cussó R, Parra J (2000) A short training programme for the rapid improvement of both aerobic and anaerobic metabolism. Eur J Appl Physiol 82:480–486. https://doi.org/10.1007/s004210000223
Mena P, Maynar M, Gutierrez JM, Maynar J, Timon J, Campillo J (1991) Erythrocyte free radical scavenger enzymes in bicycle professional racers. Adaptation to training. Int J Sports Med 12:563–566. https://doi.org/10.1055/s-2007-1024734
Clark NA, Teschke K, Rideout K, Copes R (2007) Trace element levels in adults from the west coast of Canada and associations with age, gender, diet, activities, and levels of other trace elements. Chemosphere 70:155–164. https://doi.org/10.1016/J.CHEMOSPHERE.2007.06.038
Pograjc L, Stibilj V, Falnoga I (2012) Impact of intensive physical activity on selenium status. Biol Trace Elem Res 145:291–299. https://doi.org/10.1007/s12011-011-9204-9
Rodríguez Rodríguez EM, Sanz Alaejos MT, Díaz Romero C (1995) Urinary selenium status of healthy people. Eur J Clin Chem Clin Biochem 33:127–133
Emre MH, Düzova H, Sancak B, Polat A, Erdoğan H, Yologlu S (2004) Serum selenium response to maximal anaerobic exercise among sportsmen trained at various levels. J Trace Elem Exp Med 17:93–100. https://doi.org/10.1002/jtra.20000
Margaritis I, Rousseau A-S, Hininger I, Palazzetti SÉP, Arnaud J, Roussel AM (2005) Increase in selenium requirements with physical activity loads in well-trained athletes is not linear. Biofactors 23:45–55. https://doi.org/10.1002/biof.5520230106
Micheletti A, Rossi R, Rufini S (2001) Zinc status in athletes. Sport Med 31:577–582. https://doi.org/10.2165/00007256-200131080-00002
Arikan S, Akkus H, Halifeoglu I, Baltaci AK (2008) Comparison of plasma leptin and zinc levels in elite athletes and sedentary people. Cell Biochem Funct 26:655–658. https://doi.org/10.1002/cbf.1480
Giolo De Carvalho F, Rosa FT, Marques Miguel Suen V et al (2012) Evidence of zinc deficiency in competitive swimmers. Nutrition 28:1127–1131. https://doi.org/10.1016/j.nut.2012.02.012
Lukaski HC (2005) Low dietary zinc decreases erythrocyte carbonic anhydrase activities and impairs cardiorespiratory function in men during exercise. Am J Clin Nutr 81:1045–1051
DeRuisseau KC, Cheuvront SN, Haymes EM, Sharp RG (2002) Sweat iron and zinc losses during prolonged exercise. Int J Sport Nutr Exerc Metab 12:428–437
Giugliano R, Millward DJ (1984) Growth and zinc homeostasis in the severely Zn-deficient rat. Br J Nutr 52:545. https://doi.org/10.1079/BJN19840122
Buchman AL, Keen C, Commisso J, Killip D, Ou CN, Rognerud CL, Dennis K, Dunn JK (1998) The effect of a Marathon run on plasma and urine mineral and metal concentrations. J Am Coll Nutr 17:124–127. https://doi.org/10.1080/07315724.1998.10718737
Kara E, Ozal M, Gunay M, Kilic M, Baltaci AK, Mogulkoc R (2011) Effects of exercise and zinc supplementation on cytokine release in young wrestlers. Biol Trace Elem Res 143:1435–1440. https://doi.org/10.1007/s12011-011-9005-1
Minini RAB, Laposy CB, Neto H et al (2013) Concentrações de ferro, cobre, zinco e manganês em equinos da raça Puro-sangue Lusitano, antes e após exercício. Pesqui Veterinária Bras 33:1045–1048. https://doi.org/10.1590/S0100-736X2013000800016
Tipton K, Green NR, Haymes EM, Waller M (1993) Zinc loss in sweat of athletes exercising in hot and neutral temperatures. Int J Sport Nutr 3:261–271
Volpe SL, Lowe NM, Woodhouse LR, King JC (2007) Effect of maximal exercise on the short-term kinetics of zinc metabolism in sedentary men. Br J Sports Med 41:156–161. https://doi.org/10.1136/bjsm.2006.030346
Bicer M, Akil M, Sivrikaya A, Kara E, Baltaci AK, Mogulkoc R (2011) Effect of zinc supplementation on the distribution of various elements in the serum of diabetic rats subjected to an acute swimming exercise. J Physiol Biochem 67:511–517. https://doi.org/10.1007/s13105-011-0096-0
Kaya M (2008) Comparison of urine and blood zinc levels of futsal players before and after the match. Asian J Chem 20:3203–3208
König D, Weinstock C, Keul J, Northoff H, Berg A (1998) Zinc, iron, and magnesium status in athletes—influence on the regulation of exercise-induced stress and immune function. Exerc Immunol Rev 4:2–21
Kikukawa A, Kobayashi A (2002) Changes in urinary zinc and copper with strenuous physical exercise. Aviat Space Environ Med 73:991–995
Granell J (2014) Zinc and copper changes in serum and urine after aerobic endurance and muscular strength exercise. J Sports Med Phys Fitness 54:232–237
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The authors received financial support provided by the European Regional Development Fund (ERDF) and the Government of Extremadura (project PRI08B130) as well as the collaboration of SAIUex.
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All participants were informed about the aim and procedures of the study; they gave their informed consent and participated voluntarily. The study was approved by the University of Extremadura Ethics Committee in accordance with the latest version of the Helsinki declaration for human research.
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Maynar, M., Muñoz, D., Alves, J. et al. Influence of an Acute Exercise Until Exhaustion on Serum and Urinary Concentrations of Molybdenum, Selenium, and Zinc in Athletes. Biol Trace Elem Res 186, 361–369 (2018). https://doi.org/10.1007/s12011-018-1327-9
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DOI: https://doi.org/10.1007/s12011-018-1327-9