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Fluoride-Induced Oxidative and Inflammatory Stress in Osteosarcoma Cells: Does It Affect Bone Development Pathway?

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Abstract

Oxidative stress is reported to negatively affect osteoblast cells. Present study reports oxidative and inflammatory signatures in fluoride-exposed human osteosarcoma (HOS) cells, and their possible association with the genes involved in osteoblastic differentiation and bone development pathways. HOS cells were challenged with sublethal concentration (8 mg/L) of sodium fluoride for 30 days and analyzed for transcriptomic expression. In total, 2632 transcripts associated with several biological processes were found to be differentially expressed. Specifically, genes involved in oxidative stress, inflammation, osteoblastic differentiation, and bone development pathways were found to be significantly altered. Variation in expression of key genes involved in the abovementioned pathways was validated through qPCR. Expression of serum amyloid A1 protein, a key regulator of stress and inflammatory pathways, was validated through western blot analysis. This study provides evidence that chronic oxidative and inflammatory stress may be associated with the fluoride-induced impediment in osteoblast differentiation and bone development.

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Acknowledgments

The authors are grateful to Council of Scientific and Industrial Research (CSIR), India, for providing research grant and necessary facilities under INDEPTH networking project (BSC0111). Deepa Gandhi is thankful to the Department of Science and Technology (DST), India, for the award of senior research fellowship (number IF110408). This manuscript represents CSIR-NEERI communication number KRC\2016\MAY\EHD\1.

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Correspondence to Saravanadevi Sivanesan.

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Gandhi, D., Naoghare, P.K., Bafana, A. et al. Fluoride-Induced Oxidative and Inflammatory Stress in Osteosarcoma Cells: Does It Affect Bone Development Pathway?. Biol Trace Elem Res 175, 103–111 (2017). https://doi.org/10.1007/s12011-016-0756-6

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