Abstract
Accumulation of aluminium and acrylamide in food is a major source of human exposure. Their adverse effects are well documented, but there is no information about the health problems arising from their combined exposure. The aim of the present study was to examine the possible neurotoxic effects after co-exposure of pregnant and lactating rats to aluminium and acrylamide in order to evaluate redox state, cholinergic function and membrane-bound ATPases in the cerebellum of adult rats and their progeny. Pregnant female rats have received aluminium (50 mg/kg body weight) via drinking water and acrylamide (20 mg/kg body weight) by gavage, either individually or in combination from the 14th day of pregnancy until day 14 after delivery. Exposure to these toxicants provoked an increase in malondialdehyde (MDA) and advanced oxidation protein product (AOPP) levels and a decrease in SOD, CAT, GPx, Na+K+-ATPase, Mg2+-ATPase and AChE activities in the cerebellum of mothers and their suckling pups. A reduction in GSH, NPSH and vitamin C levels was also observed. These changes were confirmed by histological results. Interestingly, co-exposure to these toxicants exhibited synergism based on physical and biochemical variables in the cerebellum of mothers and their progeny.
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Abbreviations
- AChE:
-
Acetylcholinesterase
- ACR:
-
Acrylamide
- AlCl3 :
-
Aluminium chloride
- AOPP:
-
Advanced oxidation protein product
- CAT:
-
Catalase
- DNPH:
-
Dinitrophenyl hydrazine
- DTNB:
-
5,5′-Dithiobis-2-nitrobenzoic acid
- EGL:
-
External granular layer
- GPx:
-
Glutathione peroxidase
- GSH:
-
Reduced glutathione
- IGL:
-
Internal granular layer
- MDA:
-
Malondialdehyde
- ML:
-
Molecular layer
- NPSH:
-
Non-protein thiol
- PCL:
-
Purkinje cell layer
- SOD:
-
Superoxide dismutase
- TBA:
-
Thiobarbituric acid
- TBARS:
-
Thiobarbituric acid-reactive substances;
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Acknowledgments
This work was supported by the Ministry of Higher Education and Scientific Research in Tunisia (DGRST grants, LR/11ES-53).The authors are indebted to Mr. Menaa Assili and Mr. Chedli Tmar for their skilful technical assistance.
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The general guidelines for the use and care of living animals in scientific investigations were followed (Council of European Communities: JO 86/609/CEE, 1986). The handling of the animals and the care and use of laboratory animals were approved by the Tunisian Ethical Committee of Sciences Faculty of Sfax.
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The authors declare that they have no competing interests.
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Ibtissem Ben Amara and Naourez Ktari contributed equally to this work
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Ghorbel, I., Amara, I.B., Ktari, N. et al. Aluminium and Acrylamide Disrupt Cerebellum Redox States, Cholinergic Function and Membrane-Bound ATPase in Adult Rats and Their Offspring. Biol Trace Elem Res 174, 335–346 (2016). https://doi.org/10.1007/s12011-016-0716-1
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DOI: https://doi.org/10.1007/s12011-016-0716-1