Abstract
We explored the ability of local and systemic applications of boric acid (BA) to reduce the numbers of methicillin-resistant Staphylococcus aureus (MRSA) in a rat model of tibial osteomyelitis (OM), and compared boric acid with vancomycin (V). Implant-associated osteomyelitis was established in 35 rats. After 4 weeks, at which time OM was evident both radiologically and serologically in all animals, the rats were divided into five groups of equal number: group 1, control group (no local application of BA or other medication); group 2, V group; group 3, local BA + V group; group 4, local BA group; and group 5, local + systemic BA group. Serum total antioxidant status, and the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6, were measured. Pathological changes attributable to bone OM were evaluated using a grading system. Bacterial colony-forming units (CFUs) per gram of bone were counted. The lowest bacterial numbers were evident in group 3, and the bacterial numbers were significantly lower than that of the control group in all four test groups (p < 0.001). Group 3 also had the least severe bone infection (OM score 1.7 ± 1.1, p < 0.05). Upon histological and microbiological evaluation, no significant difference was evident between groups 2 and 3. Total antioxidant levels were significantly different in all treatment groups compared to the control group. Microbiological and histopathological evaluation showed that systemic or local application of BA was effective to treat OM, although supplementary V increased the effectiveness of BA.
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No benefits in any form have been or will be receivedfroma commercial party related directly or indirectly to the subjectof this manuscript. The authors thank Havva ERDEM ( associated professor, MD, in Ordu University Medical School, Pathology Department) for contributions to hystopathological evaluation.
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Our study protocol was approved by the Animal Welfare Committee of the Medical University of Canakkale Onsekiz Mart, Canakkale, Turkey.
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Güzel, Y., Golge, U.H., Goksel, F. et al. The Efficacy of Boric Acid Used to Treat Experimental Osteomyelitis Caused by Methicillin-Resistant Staphylococcus aureus: an In Vivo Study. Biol Trace Elem Res 173, 384–389 (2016). https://doi.org/10.1007/s12011-016-0662-y
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DOI: https://doi.org/10.1007/s12011-016-0662-y