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Selenium Deficiency Affects the mRNA Expression of Inflammatory Factors and Selenoprotein Genes in the Kidneys of Broiler Chicks

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Abstract

The aim of this study was to investigate the influence of Se deficiency on the transcription of inflammatory factors and selenoprotein genes in the kidneys of broiler chicks. One hundred fifty 1-day-old broiler chicks were randomly assigned to two groups fed with either a low-Se diet (L group, 0.033 mg/kg Se) or an adequate Se diet (C group, 0.2 mg/kg Se). The levels of uric acid (UA) and creatinine (Cr) in the serum and the mRNA levels of 6 inflammatory factors and 25 selenoprotein genes in the kidneys were measured as the clinical signs of Se deficiency occurred at 20 days old. The results indicated that the contents of UA and Cr in the serum increased in L group (p < 0.05), and the mRNA levels of the inflammatory factors (NF-κB, iNOS, COX-2, and TNF-α) increased in L group (p < 0.05). Meanwhile, the mRNA levels of PTGEs and HO-1 were not changed. In addition, 25 selenoprotein transcripts displayed ubiquitous expression in the kidneys of the chicks. The mRNA levels of 14 selenoprotein genes (Dio1, Dio2, GPx3, Sepp1, SelH, SelI, SelK, Sepn1, SelO, SelW, Sep15, SelT, SelU, and SelS) decreased, and 9 selenoprotein genes (GPx1, GPx2, GPx4, SelPb, Txnrd1, Txnrd2, Txnrd3, SPS2, and SelM) increased in L group (p < 0.05), but the Dio3 and Sepx1 mRNA levels did not change. The results indicated that Se deficiency resulted in kidney dysfunction, activation of the NF-κB pathway, and a change in selenoprotein gene expression. The changes of inflammatory factor and selenoprotein gene expression levels were directly related to the abnormal renal functions induced by Se deficiency.

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Acknowledgments

This study was supported by the Research Fund for the Doctoral Program of Higher Education (20122325110018), the National Natural Science Foundations of China (31472104; 31272626; 30871902), and the National Science and Technology Support Program (2013BAD20B04). The authors thank the members in the Veterinary Internal Medicine Laboratory at the College of Veterinary Medicine, Northeast Agricultural University for their help in analyzing the data.

Conflict of Interest

All authors declare that they have no competing interests.

Compliance with Ethical Standards

All of the procedures used in this experiment were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jiu-li Zhang, Yu-hong Gao & An-shan Shan

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Bo Xu, Xiao-dan Huang & Yu Chen

  3. Heilongjiang Polytechnic, Harbin, 150080, People’s Republic of China

    Jiu-li Zhang & Yu-hong Gao

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  1. Jiu-li Zhang
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Correspondence to An-shan Shan.

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Zhang, Jl., Xu, B., Huang, Xd. et al. Selenium Deficiency Affects the mRNA Expression of Inflammatory Factors and Selenoprotein Genes in the Kidneys of Broiler Chicks. Biol Trace Elem Res 171, 201–207 (2016). https://doi.org/10.1007/s12011-015-0512-3

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