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The Selenylation Modification of Epimedium Polysaccharide and Isatis Root Polysaccharide and the Immune-enhancing Activity Comparison of Their Modifiers

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Abstract

Epimedium polysaccharide (EPS) and isatis root polysaccharide (IRPS) were extracted, purified, and selenizingly modified by nitric acid-sodium selenite method to obtain nine selenizing EPSs (sEPSs), sEPS1–sEPS9 and nine selenizing IRPSs (sIRPSs), sIRPS1–sIRPS9, respectively. Their effects on chicken peripheral lymphocyte proliferation in vitro were compared by MTT assay. The results showed that selenium polysaccharides at appropriate concentration could promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 and sIRPS5 with stronger actions were picked out and injected into the chickens vaccinated with Newcastle disease vaccine in vivo tests. The peripheral lymphocyte proliferation and serum antibody titer were determined. The results showed that sEPS5 and sIRPS5 could elevate serum antibody titer and promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 possessed the strongest efficacy. These results indicate that selenylation modification can significantly enhance the immune-enhancing activity of EPS and IRPS, and sEPS5 can be as a new-type immunopotentiator of chickens.

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Acknowledgments

The project was supported by National Natural Science Foundation of China (31272596), Special Fund for Agro-scientific Research in the Public Interest (201403051), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. We are grateful to all other staff in the Institute of Traditional Chinese Veterinary Medicine of Nanjing Agricultural University for their assistance in the experiments.

Compliance with Ethical Standards

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Conflict of Interest

The authors declare that they have no conflict of interests.

Ethical Approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the Institutional Animal Care and Use Committee of the Laboratory Animal Research Center in Nanjing Agricultural University.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People’ s Republic of China

    Xiuping Li, Ranran Hou, Chanjuan Yue, Jie Liu, Zhenzhen Gao, Deyun Wang, Cui Liu & Yuanliang Hu

  2. Department of Agriculture, Dezhou University, Dezhou, 253023, People’ s Republic of China

    Xiuping Li

  3. National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’ s Republic of China

    Jin Chen & Yu Lu

Authors
  1. Xiuping Li
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  2. Ranran Hou
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  3. Chanjuan Yue
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  4. Jie Liu
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  5. Zhenzhen Gao
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  6. Jin Chen
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  7. Yu Lu
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  8. Deyun Wang
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  9. Cui Liu
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  10. Yuanliang Hu
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Correspondence to Yuanliang Hu.

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Li, X., Hou, R., Yue, C. et al. The Selenylation Modification of Epimedium Polysaccharide and Isatis Root Polysaccharide and the Immune-enhancing Activity Comparison of Their Modifiers. Biol Trace Elem Res 171, 224–234 (2016). https://doi.org/10.1007/s12011-015-0511-4

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  • DOI: https://doi.org/10.1007/s12011-015-0511-4

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