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Mechanisms of Intracellular Calcium Homeostasis in MC3T3-E1 Cells and Bone Tissues of Sprague-Dawley Rats Exposed to Fluoride

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Abstract

Calcium homeostasis of osteoblasts (OBs) has an important role in the physiology and pathology of bone tissue. In order to study the mechanisms of intracellular calcium homeostasis, MC3T3-E1 cells and Sprague-Dawley rats were treated with different concentrations of fluoride. Then, we examined intracellular-free calcium ion ([Ca2+]i) in MC3T3-E1 cells as well as mRNA and protein levels of Cav1.2, the main subunit of L-type voltage-dependent calcium channels (VDCCs), Na+/Ca2+ exchange carriers (NCS), and plasma membrane Ca2+-ATPase (PMCA), inositol 1,4,5-trisphosphate receptor (IP3R) channels, sarco/endoplasmic reticulum calcium ATPase 2b (SERCA2b)/ATP2A2 in vitro, and rat bone tissues in vivo. Our results showed that [Ca2+]i of fluoride-treated OBs increased in a concentration-dependent manner with an increase in the concentration of fluoride. We also found that the low dose of fluoride led to high expression levels of Cav1.2, NCS-1, and PMCA and low expression levels of IP3R and SERCA2b/ATP2A2, while the high dose of fluoride induced an increase in SERCA2b/ATP2A2 levels and decrease in Cav1.2, PMCA, NCS-1, and IP3R levels. These results demonstrate that calcium channels and calcium pumps of plasma and endoplasmic reticulum (ER) membranes keep intracellular calcium homeostasis by regulating Cav1.2, NCS-1, PMCA, IP3R, and SERCA2b/ATP2A2 expression.

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Acknowledgments

This work was supported by a grant for skeletal fluorosis research from the National Natural Science Foundation of China (81172608).

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. The Second Hospital Norman Bethune of Jilin University, Changchun, 130041, People’s Republic of China

    Xiao-qin Duan, Yan-hui Li & Huan Wang

  2. Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People’s Republic of China

    Xiu-yun Zhang, Zhi-tao Zhao, Guang-sheng Li & Ling Jing

  3. The First Hospital Norman Bethune of Jilin University, Changchun, 130021, People’s Republic of China

    Ying Wang

Authors
  1. Xiao-qin Duan
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  2. Yan-hui Li
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  3. Xiu-yun Zhang
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  4. Zhi-tao Zhao
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  5. Ying Wang
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  6. Huan Wang
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  7. Guang-sheng Li
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  8. Ling Jing
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Correspondence to Ling Jing.

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Duan, Xq., Li, Yh., Zhang, Xy. et al. Mechanisms of Intracellular Calcium Homeostasis in MC3T3-E1 Cells and Bone Tissues of Sprague-Dawley Rats Exposed to Fluoride. Biol Trace Elem Res 170, 331–339 (2016). https://doi.org/10.1007/s12011-015-0465-6

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  • DOI: https://doi.org/10.1007/s12011-015-0465-6

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