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The Effects of Ce on the Proliferation, Osteogenic Differentiation and Mineralization Function of MC3T3-E1 Cells In Vitro

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Abstract

The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of a murine preosteoblast cell line MC3T3-E1 in vitro were investigated at cell and molecular levels. The results showed that Ce promoted the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells at concentrations of 0.0001, 0.001, 0.01, 0.1 and 1 μM, but turned to inhibit the proliferation, osteogenic differentiation and mineralization function at concentrations of 10, 100 and 1000 μM. Ce displayed the up-regulation of Runx2, BMP2, ALP, BSP, Col I and OCN genes at concentrations of 0.0001 and 0.1 μM; these genes were down-regulated in the MC3T3-E1 cells treated with 1000 μM Ce. The expression of BMP2, Runx2 and OCN proteins was promoted by Ce at concentrations of 0.0001 and 0.1 μM, but these proteins were down-regulated after 1000 μM Ce treatment. The results suggest that Ce likely up-regulates or down-regulates the expression of Runx2, which subsequently up- or down-regulates OB marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causing to promote or inhibit the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells.

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Abbreviations

Q-PCR:

Quantitative real-time reverse transcriptase polymerase chain reaction

Runx2:

Runt-related transcription factor 2

BMP2:

Bone morphogenetic protein 2

TGF-β:

Transforming growth factor-β

ALP:

Alkaline phosphatase

BSP:

Bone sialoprotein

Col I:

Collagen I

OCN:

Osteocalcin

Ln:

Lanthanides

PBS:

Phosphate buffer saline

ARS:

Alizarin red S

OBs:

Osteoblasts

ATCC:

American Type Culture Collection

α-MEM:

Alpha minimum essential medium

FBS:

Fatal bovine serum

EDTA:

Ethylenediaminetetraacetic acid tetrasodium salt

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

DMSO:

Dimethylsulfoxide

OD:

Optical density

OS:

Osteogenetic induction supplement

ECL:

Enhanced chemiluminescence

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

SD:

Standard deviation

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20971034), Natural Science Key Foundation of Hebei Province (No. B2009000161) and Research Fund for the Doctoral Program of Higher Education of China (No. 20111301110004).

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People’s Republic of China

    Dandan Liu, Jinchao Zhang, Yaping Li & Shuxiang Wang

  2. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, People’s Republic of China

    MengSu Yang

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  1. Dandan Liu
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  2. Jinchao Zhang
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  4. Shuxiang Wang
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Correspondence to Jinchao Zhang.

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Liu, D., Zhang, J., Li, Y. et al. The Effects of Ce on the Proliferation, Osteogenic Differentiation and Mineralization Function of MC3T3-E1 Cells In Vitro. Biol Trace Elem Res 149, 291–297 (2012). https://doi.org/10.1007/s12011-012-9423-8

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  • DOI: https://doi.org/10.1007/s12011-012-9423-8

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