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CDCA8 Facilitates Tumor Proliferation and Predicts a Poor Prognosis in Hepatocellular Carcinoma

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Abstract

CDCA8 expression is abnormally high in a variety of cancers and involved in the biological process of tumor malignancy. In this study, we discovered that the expression of CDCA8 was up-regulated in hepatocellular carcinoma cancer (HCC) tissues and high levels of CDCA8 are associated with larger tumor size, higher AFP (α-fetoprotein) levels, and unfavorable prognosis. Cell functional experiments revealed that CDCA8 silencing remarkably inhibited proliferation and promoted apoptosis in SNU-387 and Hep-3B cells. The results of flow cytometry showed that CDCA8 regulated CDK1 and cyclin B1 expression to arrest at the S phase, inhibited proliferation, and promoted apoptosis. In addition, in vivo studies have confirmed that silencing CDCA8 could regulate CDK1/cyclin B1 signaling axis to inhibit the growth of HCC xenograft tumor. Our study demonstrated CDCA8 acts an oncogene to facilitate cell proliferation of HCC via regulating cell cycle, indicating the promising application value of CDCA8 for HCC diagnosis and clinical treatment.

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All data and materials in the manuscript will be made available by the corresponding authors.

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Acknowledgements

We are grateful to all participates for their contributions for the present study.

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute & Hospital, Tianjin, China

    Yunlong Cui

  2. Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Tianjin University of Sport, No.16 Donghai Road, West Tuanbo New Town, Jinghai District, Tianjin, China

    Ning Jiang

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  1. Yunlong Cui
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  2. Ning Jiang
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Contributions

Y. C.: Writing, conceptualization, methodology, software

N. J.: Data curation, writing, original draft preparation, and editing the manuscript

Corresponding author

Correspondence to Ning Jiang.

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This study was approved by the ethics committee of Tianjin Medical University Cancer Institute & Hospital and written consent approval was obtained from all participants.

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The authors declare no competing interests.

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Cui, Y., Jiang, N. CDCA8 Facilitates Tumor Proliferation and Predicts a Poor Prognosis in Hepatocellular Carcinoma. Appl Biochem Biotechnol 196, 1481–1492 (2024). https://doi.org/10.1007/s12010-023-04603-w

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