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MIP-Based Impedimetric Sensor for Detecting Dengue Fever Biomarker

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Abstract

In this study, molecular imprinted polymer (MIP)-based impedimetric sensor has been developed to detect dengue infection at an early stage. Screen-printed carbon electrode (SPCE) was modified with electrospun nanofibers of polysulfone (PS) and then, coated with dopamine while using NS1 (non-structural protein 1—a specific and sensitive biomarker for dengue virus infection) as template during polymerization. The self-polymerization of dopamine at room temperature helps to retain exact structure of template (NS1) which results in generating geometrically fit imprinted sites for specific detection of target analyte. The electrochemical properties of MIP-modified SPCEs were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) at every step of modification. Under optimal conditions, impedimetric measurements showed linear response in the range from 1 to 200 ng/mL. The developed sensor can selectively detect NS1 concentrations as low as 0.3 ng/mL. Moreover, impedimetric sensor system was also employed for NS1 determination in real human serum samples and satisfying recoveries varying from 95 to 97.14% were obtained with standard deviations of less than 5%.

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Funding

The authors are thankful to the Higher Education Commission (HEC) of Pakistan for financial support under project no. 5411.

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Authors and Affiliations

  1. Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore, 54600, Pakistan

    Rabia Arshad, Akhtar Hayat, Mian Hasnain Nawaz & Usman Latif

  2. Ecole Nationale Supérieure de Biotechnologie, 25100, Constantine, Algeria

    Amina Rhouati

  3. Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, 22060, Pakistan

    Muhammad Arfat Yameen

  4. Institute of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan

    Adnan Mujahid

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  1. Rabia Arshad
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  2. Amina Rhouati
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  3. Akhtar Hayat
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  4. Mian Hasnain Nawaz
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Correspondence to Usman Latif.

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Arshad, R., Rhouati, A., Hayat, A. et al. MIP-Based Impedimetric Sensor for Detecting Dengue Fever Biomarker. Appl Biochem Biotechnol 191, 1384–1394 (2020). https://doi.org/10.1007/s12010-020-03285-y

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