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Phytase Immobilization on Hydroxyapatite Nanoparticles Improves Its Properties for Use in Animal Feed

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Abstract

The enzyme phytase has important applications in animal feed, because it favors the bioavailability of phosphorus present in phytate, an antinutritional compound widely found associated with plant proteins. However, for feed applications, the phytase must withstand high temperatures during the feed pelleting process, as well as the gastrointestinal conditions of the animal. This work evaluates the feasibility of immobilizing phytase on hydroxyapatite (HA) nanoparticles, in order to improve its properties. HA is a material with excellent physicochemical characteristics for enzyme immobilization, and it can also act as an inorganic source of phosphorus and calcium in animal feed. The strong affinity of the phytase for the support resulted in rapid adsorption, with total immobilization yield and recovered activity greater than 100%. After immobilization, the phytase showed a broader activity profile in terms of pH and temperature, together with considerably higher thermoresistance at 80 and 90 °C. As a proof of concept, it was shown that the phytase immobilized on HA presented good resistance to acidic conditions and resistance to proteolysis when passing through simulated gastrointestinal conditions of fish. The findings showed that phytase immobilized onto HA presents suitable properties and has great potential for use in animal feed.

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Change history

  • 25 October 2019

    In the original version of this article, under <Emphasis Type="Bold">Calculation of Immobilization Parameters</Emphasis> heading, the presentation of the equations are incorrect. The correct presentation of the equations are given below:

  • 25 October 2019

    In��the original version of this article, under Calculation of Immobilization Parameters heading, the presentation of the equations are incorrect. The correct presentation of the equations are given below:

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Funding

Embrapa, CNPq (Process 401182/2014-2), CAPES, and FAPESP (Process 2016/10636-8) (all from Brazil) provided financial support.

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  1. Embrapa Instrumentation, Rua XV de Novembro 1452, Sao Carlos, SP, 13560-970, Brazil

    Thamara C. Coutinho & Cristiane S. Farinas

  2. Graduate Program of Chemical Engineering, Federal University of São Carlos, Sao Carlos, SP, 13565-905, Brazil

    Thamara C. Coutinho, Paulo W. Tardioli & Cristiane S. Farinas

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  1. Thamara C. Coutinho
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Coutinho, T.C., Tardioli, P.W. & Farinas, C.S. Phytase Immobilization on Hydroxyapatite Nanoparticles Improves Its Properties for Use in Animal Feed. Appl Biochem Biotechnol 190, 270–292 (2020). https://doi.org/10.1007/s12010-019-03116-9

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