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Efficient Production of 2,6-Difluorobenzamide by Recombinant Escherichia coli Expressing the Aurantimonas manganoxydans Nitrile Hydratase

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Abstract

2,6-Difluorobenzamide is an important intermediate with many applications in pesticide industries. Through screening a library of recombinant nitrile hydratases, the nitrile hydratase from Aurantimonas manganoxydans ATCC BAA-1229 was selected for production of 2,6-difluorobenzamide from 2,6-difluorobenzonitrile. Key parameters of the biocatalytic process, including temperature, pH, substrate loading, and substrate feeding mode, were optimized. Finally, 314 g/L of 2,6-difluorobenzamide was produced in a simple batch process within 11 h without formation of any by-product in an economical non-buffer system and similar result was obtained when scaled up to 30 L. This study constitutes the first report of 2,6-difluorobenzamide significant production using a recombinant Escherichia coli-based biocatalyst.

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Funding

This work was supported by the National Natural Science Foundation of China (Nos. 21476199, 21676240).

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Authors and Affiliations

  1. Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China

    Zhengfei Yang, Gang Xu, Jianping Wu & Lirong Yang

  2. College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310012, People’s Republic of China

    Xiaolin Pei

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  1. Zhengfei Yang
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  2. Xiaolin Pei
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  3. Gang Xu
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  5. Lirong Yang
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Correspondence to Lirong Yang.

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Yang, Z., Pei, X., Xu, G. et al. Efficient Production of 2,6-Difluorobenzamide by Recombinant Escherichia coli Expressing the Aurantimonas manganoxydans Nitrile Hydratase. Appl Biochem Biotechnol 187, 439–448 (2019). https://doi.org/10.1007/s12010-018-2823-2

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  • DOI: https://doi.org/10.1007/s12010-018-2823-2

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